David S Watt | Molecular & Cellular Biochemistry

David S Watt

dwatt@uky.edu

859-576-7373

Todd 421

Position(s):

Professor

Director of the Organic Synthesis Core

Affiliation(s):

Molecular & Cellular Biochemistry

Center for Molecular Medicine - Organic Synthesis Core

Other Affiliation(s):

College of Pharmacy Department of Pharmaceutical Sciences

College of Pharmacy

Bio / Education:

A.B., Dartmouth College, Hanover, NH, 1967
Ph.D, Harvard University, Cambridge, MA, 1972 with Professor E. J. Corey
NIH Postdoctoral Fellow, Biochemistry, Harvard University, Cambridge, MA, 1972-73 with Professor Konrad Bloch

Research Description:

Overview

My laboratory has a long-standing research interest in problems at the interface of biology and chemistry. We provide access to novel compounds that are not available through commercial sources. Recent efforts include small-molecule inhibitors for enzymatic reactions, fluorescent derivatives of natural products and semisynthetic natural products, and unnatural peptides that would have better stability than wild-type counterparts.

Recent highlights involve work in the areas of cancer, infectious diseases and neuroscience, as summarized in our recent publications.

Selected Publications:

Shrestha, SK; Kril, LM; Green, KD; Kwiatkowski, S; Sviripa, VM; Nickell, JR; Dwoskin, LP; Watt, DS; Garneau-Tsodikova, S (2017) Bis(N-amidinohydrazones) and N-(amidino)-N'-aryl-bishydrazones: New classes of antibacterial/antifungal agents Bioorg Med Chem 25(1):58-66. PMID: 27769670. PMCID: PMC5164969.
Frasinyuk, MS; Zhang, W; Wyrebek, P; Yu, T; Xu, X; Sviripa, VM; Bondarenko, SP; Xie, Y; Ngo, NG; Morris, AJ; Mohler, JL; Fiandalo, MV; Watt, DS; and Liu, C (2017) Developing Antineoplastic Agents that Targeting Peroxisomal Enzymes: Cytisine-linked Isoflavonoids as Inhibitors of Hydroxysteroid 17-beta-dehydrogenase-4 (HSD17B4) Org Biomol Chem 15:7623-7629. PMID: 28868548; PMCID: PMC5607111
Kril, LM; Xie, Y; Zhang, W; Wyrebek P; Liu, X; Watt, DS; Liu, C; Sviripa, VM b,e,* Phenylethynyl-substituted Heterocycles Inhibit Cyclin D1 and Induce the Expression of Cyclin-dependent Kinase Inhibitor p21Wif1/Cip1 in Colorectal Cancer Cells (2018) MedChemComm, in press. http://dx.doi.org/10.1039/C7MD00393E.

PubMed Publications:

Howard, KC ; Dennis, EK ; Watt, DS ; Garneau-Tsodikova, S. "A comprehensive overview of the medicinal chemistry of antifungal drugs: perspectives and promise." Chemical Society reviews (2020): [PubMed Link] | [ Full text ]
Tang, B.; Frasinyuk, MS ; Chikwana, VM ; Mahalingan, KK ; Morgan, CA ; Segvich, DM ; Bondarenko, SP ; Mrug, GP ; Wyrebek, P.; Watt, DS ; DePaoli-Roach, AA ; Roach, PJ ; Hurley, TD "Discovery and Development of Small-Molecule Inhibitors of Glycogen Synthase." Journal of medicinal chemistry (2020): [PubMed Link] | [ Full text ]
Chandrika, Thamban ; Dennis, EK ; Shrestha, SK ; Ngo, HX ; Green, KD ; Kwiatkowski, S.; Deaciuc, AG ; Dwoskin, LP ; Watt, DS ; Garneau-Tsodikova, S. "N,N'-diaryl-bishydrazones in a biphenyl platform: Broad spectrum antifungal agents." European journal of medicinal chemistry 164, (2019): 273-281. [PubMed Link] | [ Full text ]
Zhang, W.; Sviripa, VM ; Kril, LM ; Yu, T.; Xie, Y.; Hubbard, WB ; Sullivan, PG ; Chen, X.; Zhan, CG ; Yang-Hartwich, Y ; Evers, BM ; Spear, BT ; Gedaly, R.; Watt, DS ; Liu, C. "An Underlying Mechanism of Dual Wnt Inhibition and AMPK Activation: Mitochondrial Uncouplers Masquerading as Wnt Inhibitors." Journal of medicinal chemistry 62, 24 (2019): 11348-11358. [PubMed Link] | [ Full text ]
Xie, Y.; Kril, LM ; Yu, T.; Zhang, W.; Frasinyuk, MS ; Bondarenko, SP ; Kondratyuk, KM ; Hausman, E ; Martin, ZM ; Wyrebek, PP ; Liu, X.; Deaciuc, A ; Dwoskin, LP ; Chen, J.; Zhu, H.; Zhan, CG ; Sviripa, VM ; Blackburn, J.; Watt, DS ; Liu, C. "Semisynthetic aurones inhibit tubulin polymerization at the colchicine-binding site and repress PC-3 tumor xenografts in nude mice and myc-induced T-ALL in zebrafish." Scientific reports 9, 1 (2019): 6439. [PubMed Link] | [ Full text ]
Turcios, L.; Chacon, E ; García, C.; Eman, P ; Cornea, V.; Jiang, J.; Spear, B.; Liu, C.; Watt, DS ; Marti, F.; Gedaly, R. "Autophagic flux modulation by Wnt/β-catenin pathway inhibition in hepatocellular carcinoma." PloS one 14, 2 (2019): e0212538. [PubMed Link] | [ Full text ]
Johnson, J.; Rychahou, P.; Sviripa, VM ; Weiss, HL ; Liu, C.; Watt, DS ; Evers, BM "Induction of AMPK activation by N,N'-diarylurea FND-4b decreases growth and increases apoptosis in triple negative and estrogen-receptor positive breast cancers." PloS one 14, 3 (2019): e0209392. [PubMed Link] | [ Full text ]
Sinner, HF ; Johnson, J.; Rychahou, PG ; Watt, DS ; Zaytseva, YY ; Liu, C.; Evers, BM "Novel chemotherapeutic agent, FND-4b, activates AMPK and inhibits colorectal cancer cell proliferation." PloS one 14, 10 (2019): e0224253. [PubMed Link] | [ Full text ]
Cook, SF ; Fiandalo, MV ; Watt, DS ; Wu, Y.; Mohler, JL ; Bies, RR "Mathematical modeling of intracrine androgen metabolism in prostate cancer: Methodological aspects." The Prostate (2018): [PubMed Link] | [ Full text ]
Fiandalo, MV ; Stocking, JJ ; Pop, EA ; Wilton, JH ; Mantione, KM ; Li, Y.; Attwood, KM ; Azabdaftari, G.; Wu, Y.; Watt, DS ; Wilson, EM ; Mohler, JL "Inhibition of dihydrotestosterone synthesis in prostate cancer by combined frontdoor and backdoor pathway blockade." Oncotarget 9, 13 (2018): 11227-11242. [PubMed Link] | [ Full text ]
Sviripa, VM ; Kril, LM ; Zhang, W.; Xie, Y.; Wyrebek, P.; Ponomareva, L.; Liu, X.; Yuan, Y.; Zhan, CG ; Watt, DS ; Liu, C. "Phenylethynyl-substituted Heterocycles Inhibit Cyclin D1 and Induce the Expression of Cyclin-dependent Kinase Inhibitor p21Wif1/Cip1 in Colorectal Cancer Cells." MedChemComm 9, 1 (2018): 87-99. [PubMed Link] | [ Full text ]
Frasinyuk, MS ; Zhang, W.; Wyrebek, P.; Yu, T.; Xu, X.; Sviripa, VM ; Bondarenko, SP ; Xie, Y.; Ngo, HX ; Morris, AJ ; Mohler, JL ; Fiandalo, MV ; Watt, DS ; Liu, C. "Developing antineoplastic agents that target peroxisomal enzymes: cytisine-linked isoflavonoids as inhibitors of hydroxysteroid 17-beta-dehydrogenase-4 (HSD17B4)." Organic & biomolecular chemistry 15, 36 (2017): 7623-7629. [PubMed Link] | [ Full text ]
Kenlan, DE ; Rychahou, P.; Sviripa, VM ; Weiss, HL ; Liu, C.; Watt, DS ; Evers, BM "Fluorinated N,N'-Diarylureas As Novel Therapeutic Agents Against Cancer Stem Cells." Molecular cancer therapeutics 16, 5 (2017): 831-837. [PubMed Link] | [ Full text ]
Burikhanov, R.; Hebbar, N.; Noothi, SK ; Shukla, N.; Sledziona, J.; Araujo, N.; Kudrimoti, M.; Wang, QJ ; Watt, DS ; Welch, DR ; Maranchie, J.; Harada, A.; Rangnekar, VM "Chloroquine-Inducible Par-4 Secretion Is Essential for Tumor Cell Apoptosis and Inhibition of Metastasis." Cell reports 18, 2 (2017): 508-519. [PubMed Link] | [ Full text ]
Shrestha, SK ; Kril, LM ; Green, KD ; Kwiatkowski, S.; Sviripa, VM ; Nickell, JR ; Dwoskin, LP ; Watt, DS ; Garneau-Tsodikova, S. "Bis(N-amidinohydrazones) and N-(amidino)-N'-aryl-bishydrazones: New classes of antibacterial/antifungal agents." Bioorganic & medicinal chemistry 25, 1 (2017): 58-66. [PubMed Link] | [ Full text ]
Frasinyuk, MS ; Mrug, GP ; Bondarenko, SP ; Khilya, VP ; Sviripa, VM ; Syrotchuk, OA ; Zhang, W.; Cai, X.; Fiandalo, MV ; Mohler, JL ; Liu, C.; Watt, DS "Antineoplastic Isoflavonoids Derived from Intermediate ortho-Quinone Methides Generated from Mannich Bases." ChemMedChem 11, 6 (2016): 600-11. [PubMed Link] | [ Full text ]
Sviripa, VM ; Burikhanov, R.; Obiero, JM ; Yuan, Y.; Nickell, JR ; Dwoskin, LP ; Zhan, CG ; Liu, C.; Tsodikov, OV ; Rangnekar, VM ; Watt, DS "Par-4 secretion: stoichiometry of 3-arylquinoline binding to vimentin." Organic & biomolecular chemistry 14, 1 (2016): 74-84. [PubMed Link] | [ Full text ]
Xu, X.; Watt, DS ; Liu, C. "Multifaceted roles for thymine DNA glycosylase in embryonic development and human carcinogenesis." Acta biochimica et biophysica Sinica 48, 1 (2016): 82-9. [PubMed Link] | [ Full text ]
Pumphrey, A.; Yang, Z.; Ye, S.; Powell, DK ; Thalman, S.; Watt, DS ; Abdel-Latif, A.; Unrine, J.; Thompson, K.; Fornwalt, B.; Ferrauto, G.; Vandsburger, M. "Advanced cardiac chemical exchange saturation transfer (cardioCEST) MRI for in vivo cell tracking and metabolic imaging." NMR in biomedicine 29, 1 (2016): 74-83. [PubMed Link] | [ Full text ]
Kwiatkowski, S.; Sviripa, VM ; Zhang, Z.; Wendlandt, AE ; Höbartner, C.; Watt, DS ; Stamm, S. "Synthesis of a norcantharidin-tethered guanosine: Protein phosphatase-1 inhibitors that change alternative splicing." Bioorganic & medicinal chemistry letters 26, 3 (2016): 965-968. [PubMed Link] | [ Full text ]
Kril, LM ; Vilchez, V.; Jiang, J.; Turcios, L.; Chen, C.; Sviripa, VM ; Zhang, W.; Liu, C.; Spear, B.; Watt, DS ; Gedaly, R. "N-Aryl benzenesulfonamide inhibitors of 3H -thymidine incorporation and β-catenin signaling in human hepatocyte-derived Huh-7 carcinoma cells." Bioorganic & medicinal chemistry letters 25, 18 (2015): 3897-9. [PubMed Link] | [ Full text ]
Yeo, SC ; Sviripa, VM ; Huang, M.; Kril, L.; Watt, DS ; Liu, C.; Lin, HS "Analysis of trans-2,6-difluoro-4'-(N,N-dimethylamino)stilbene (DFS) in biological samples by liquid chromatography-tandem mass spectrometry: metabolite identification and pharmacokinetics." Analytical and bioanalytical chemistry 407, 24 (2015): 7319-32. [PubMed Link] | [ Full text ]
Frasinyuk, MS ; Bondarenko, SP ; Sviripa, VM ; Burikhanov, R.; Rangnekar, VM ; Liu, C.; Watt, DS "Development of 6H-Chromeno 3,4-c pyrido 3',2':4,5 thieno 2,3-e pyridazin-6-ones as Par-4 Secretagogues." Tetrahedron letters 56, 23 (2015): 3382-3384. [PubMed Link] |
Frasinyuk, MS ; Bondarenko, SP ; Khilya, VP ; Liu, C.; Watt, DS ; Sviripa, VM "Synthesis and tautomerization of hydroxylated isoflavones bearing heterocyclic hemi-aminals." Organic & biomolecular chemistry 13, 4 (2015): 1053-67. [PubMed Link] | [ Full text ]
Frasinyuk, MS ; Mrug, GP ; Bondarenko, SP ; Sviripa, VM ; Zhang, W.; Cai, X.; Fiandalo, MV ; Mohler, JL ; Liu, C.; Watt, DS "Application of Mannich bases to the synthesis of hydroxymethylated isoflavonoids as potential antineoplastic agents." Organic & biomolecular chemistry 13, 46 (2015): 11292-301. [PubMed Link] | [ Full text ]
Hamza, A.; Wagner, JM ; Wei, NN ; Kwiatkowski, S.; Zhan, CG ; Watt, DS ; Korotkov, KV "Application of the 4D fingerprint method with a robust scoring function for scaffold-hopping and drug repurposing strategies." Journal of chemical information and modeling 54, 10 (2014): 2834-45. [PubMed Link] | [ Full text ]
Burikhanov, R.; Sviripa, VM ; Hebbar, N.; Zhang, W.; Layton, WJ ; Hamza, A.; Zhan, CG ; Watt, DS ; Liu, C.; Rangnekar, VM "Arylquins target vimentin to trigger Par-4 secretion for tumor cell apoptosis." Nature chemical biology 10, 11 (2014): 924-926. [PubMed Link] | [ Full text ]
Sviripa, VM ; Zhang, W.; Balia, AG ; Tsodikov, OV ; Nickell, JR ; Gizard, F.; Yu, T.; Lee, EY ; Dwoskin, LP ; Liu, C.; Watt, DS "2',6'-Dihalostyrylanilines, pyridines, and pyrimidines for the inhibition of the catalytic subunit of methionine S-adenosyltransferase-2." Journal of medicinal chemistry 57, 14 (2014): 6083-91. [PubMed Link] | [ Full text ]
Matveev, SV ; Kwiatkowski, S.; Sviripa, VM ; Fazio, RC ; Watt, DS ; Levine H, 3rd "Tritium-labeled (E,E)-2,5-bis(4'-hydroxy-3'-carboxystyryl)benzene as a probe for β-amyloid fibrils." Bioorganic & medicinal chemistry letters 24, 23 (2014): 5534-6. [PubMed Link] | [ Full text ]
Sviripa, VM ; Zhang, W.; Kril, LM ; Liu, AX ; Yuan, Y.; Zhan, CG ; Liu, C.; Watt, DS "Halogenated diarylacetylenes repress c-myc expression in cancer cells." Bioorganic & medicinal chemistry letters 24, 15 (2014): 3638-40. [PubMed Link] | [ Full text ]
Frasinyuk, MS ; Kwiatkowski, S.; Wagner, JM ; Evans, TJ ; Reed, RW ; Korotkov, KV ; Watt, DS "Pentapeptide boronic acid inhibitors of Mycobacterium tuberculosis MycP1 protease." Bioorganic & medicinal chemistry letters 24, 15 (2014): 3546-8. [PubMed Link] | [ Full text ]
Hamza, A.; Wagner, JM ; Evans, TJ ; Frasinyuk, MS ; Kwiatkowski, S.; Zhan, CG ; Watt, DS ; Korotkov, KV "Novel mycosin protease MycP₁ inhibitors identified by virtual screening and 4D fingerprints." Journal of chemical information and modeling 54, 4 (2014): 1166-73. [PubMed Link] | [ Full text ]
Sviripa, V.; Zhang, W.; Conroy, MD ; Schmidt, ES ; Liu, AX ; Truong, J.; Liu, C.; Watt, DS "Fluorinated N,N'-diarylureas as AMPK activators." Bioorganic & medicinal chemistry letters 23, 6 (2013): 1600-3. [PubMed Link] | [ Full text ]
Lin, HS ; Sviripa, VM ; Watt, DS ; Liu, C.; Xiang, TX ; Anderson, BD ; Ong, PS ; Ho, PC "Quantification of trans-2,6-difluoro-4'-N,N-dimethylaminostilbene in rat plasma: application to a pharmacokinetic study." Journal of pharmaceutical and biomedical analysis 72, (2013): 115-20. [PubMed Link] | [ Full text ]
Yeo, YS ; Nybo, SE ; Chittiboyina, AG ; Weerasooriya, AD ; Wang, YH ; Gongora-Castillo, E.; Vaillancourt, B.; Buell, CR ; Dellapenna, D.; Celiz, MD ; Jones, AD ; Wurtele, ES ; Ransom, N.; Dudareva, N.; Shaaban, KA ; Tibrewal, N.; Chandra, S.; Smillie, T.; Khan, IA ; Coates, RM ; Watt, DS ; Chappell, J. "Functional identification of valerena-1,10-diene synthase, a terpene synthase catalyzing a unique chemical cascade in the biosynthesis of biologically active sesquiterpenes in Valeriana officinalis." The Journal of biological chemistry 288, 5 (2013): 3163-73. [PubMed Link] | [ Full text ]
Zhang, W.; Sviripa, V.; Chen, X.; Shi, J.; Yu, T.; Hamza, A.; Ward, ND ; Kril, LM ; Vander Kooi, CW ; Zhan, CG ; Evers, BM ; Watt, DS ; Liu, C. "Fluorinated N,N-dialkylaminostilbenes repress colon cancer by targeting methionine S-adenosyltransferase 2A." ACS chemical biology 8, 4 (2013): 796-803. [PubMed Link] | [ Full text ]
Zhang, Z.; Kelemen, O.; Santen, MA ; Yelton, SM ; Wendlandt, AE ; Sviripa, VM ; Bollen, M.; Beullens, M.; Urlaub, H.; Lührmann, R.; Watt, DS ; Stamm, S. "Synthesis and characterization of pseudocantharidins, novel phosphatase modulators that promote the inclusion of exon 7 into the SMN (survival of motoneuron) pre-mRNA." The Journal of biological chemistry 286, 12 (2011): 10126-36. [PubMed Link] | [ Full text ]
Zhang, W.; Sviripa, V.; Kril, LM ; Chen, X.; Yu, T.; Shi, J.; Rychahou, P.; Evers, BM ; Watt, DS ; Liu, C. "Fluorinated N,N-dialkylaminostilbenes for Wnt pathway inhibition and colon cancer repression." Journal of medicinal chemistry 54, 5 (2011): 1288-97. [PubMed Link] | [ Full text ]
Niehaus, TD ; Okada, S.; Devarenne, TP ; Watt, DS ; Sviripa, V.; Chappell, J. "Identification of unique mechanisms for triterpene biosynthesis in Botryococcus braunii." Proceedings of the National Academy of Sciences of the United States of America 108, 30 (2011): 12260-5. [PubMed Link] | [ Full text ]
Wendlandt, AE ; Yelton, SM ; Lou, D.; Watt, DS ; Noonan, DJ "Synthesis and functional analysis of novel bivalent estrogens." Steroids 75, 12 (2010): 825-33. [PubMed Link] | [ Full text ]
Sumanasekera, C.; Watt, DS ; Stamm, S. "Substances that can change alternative splice-site selection." Biochemical Society transactions 36, Pt 3 (2008): 483-90. [PubMed Link] | [ Full text ]
Watt, KD ; Burak, KW ; Deschênes, M.; Lilly, L.; Marleau, D.; Marotta, P.; Mason, A.; Peltekian, KM ; Renner, E.; Yoshida, EM ; Canadian Transplant Hepatology Outcomes Research, Network. "Survival after liver transplantation for hepatitis C is unchanged over two decades in Canada." Canadian journal of gastroenterology = Journal canadien de gastroenterologie 22, 2 (2008): 153-4. [PubMed Link] |
Rajagopalan, K.; Watt, DS ; Haley, BE "Orientation of GTP and ADP within their respective binding sites in glutamate dehydrogenase." European journal of biochemistry 265, 2 (1999): 564-71. [PubMed Link] | [ Full text ]
Sams, RA ; Gerken, DF ; Detra, RL ; Stanley, SD ; Wood, WE ; Tobin, T.; Yang, JM ; Tai, HH ; Jegananthan, A.; Watt, DS "Identification of metabolites of azaperone in horse urine." Journal of pharmaceutical sciences 85, 1 (1996): 79-84. [PubMed Link] | [ Full text ]
Brock, CP ; DeLaLuz, PJ ; Golinski, M.; Lloyd, MA ; Vanaman, TC ; Watt, DS "Planarity of nitro-substituted phenothiazines." Acta crystallographica. Section B, Structural science 52 ( Pt 4), (1996): 713-9. [PubMed Link] |
Golinski, M.; DeLaLuz, PJ ; Delcamp, TJ ; Watt, DS ; Vanaman, TC "Synthesis and binding affinity of bidentate phenothiazines with two different photoactive groups." Bioconjugate chemistry 6, 5 (1995): 567-72. [PubMed Link] |
DeLaLuz, PJ ; Golinski, M.; Watt, DS ; Vanaman, TC "Synthesis and use of a biotinylated 3-azidophenothiazine to photolabel both amino- and carboxyl-terminal sites in calmodulin." Bioconjugate chemistry 6, 5 (1995): 558-66. [PubMed Link] |
Golinski, M.; DeLaLuz, PJ ; Floresca, R.; Delcamp, TJ ; Vanaman, TC ; Watt, DS "Synthesis, binding affinity, and cross-linking of monodentate photoactive phenothiazines to calmodulin." Bioconjugate chemistry 6, 5 (1995): 549-57. [PubMed Link] |
Chavan, AJ ; Richardson, SK ; Kim, H.; Haley, BE ; Watt, DS "Forskolin photoaffinity probes for the evaluation of tubulin binding sites." Bioconjugate chemistry 4, 4 (1993): 268-74. [PubMed Link] |
Salvucci, ME ; Rajagopalan, K.; Sievert, G.; Haley, BE ; Watt, DS "Photoaffinity labeling of ribulose-1,5-bisphosphate carboxylase/oxygenase activase with ATP gamma-benzophenone. Identification of the ATP gamma-phosphate binding domain." The Journal of biological chemistry 268, 19 (1993): 14239-44. [PubMed Link] | [ Full text ]
Rajagopalan, K.; Chavan, AJ ; Haley, BE ; Watt, DS "Synthesis and application of bidentate photoaffinity cross-linking reagents. Nucleotide photoaffinity probes with two photoactive groups." The Journal of biological chemistry 268, 19 (1993): 14230-8. [PubMed Link] | [ Full text ]
Dwyer, LD ; Crocker, PJ ; Watt, DS ; Vanaman, TC "The effects of calcium site occupancy and reagent length on reactivity of calmodulin lysyl residues with heterobifunctional aryl azides. Mapping interaction domains with specific calmodulin photoprobe derivatives." The Journal of biological chemistry 267, 31 (1992): 22606-15. [PubMed Link] | [ Full text ]
Drake, RR ; Slama, JT ; Wall, KA ; Abramova, M.; D'Souza, C.; Elbein, AD ; Crocker, PJ ; Watt, DS "Application of an N-(4-azido-2,3,5,6-tetrafluorobenzoyl)tyrosine-substituted peptide as a heterobifunctional cross-linking agent in a study of protein O-glycosylation in yeast." Bioconjugate chemistry 3, 1 (1992): 69-73. [PubMed Link] |
Golinski, M.; Heine, M.; Orlicky, DJ ; Fitz, TA ; Watt, DS "Prostaglandin photoaffinity probes: synthesis and binding affinity of aryl azide-substituted C-1 esters of prostaglandin F2 alpha." Eicosanoids 5, 2 (1992): 99-107. [PubMed Link] |
Golinski, M.; Heine, M.; Orlicky, DJ ; Fitz, TA ; Watt, DS "Prostaglandin photoaffinity probes: synthesis and binding affinity of C-18 substituted PGF2 alpha prostanoids bearing a perfluorinated aryl azide." Eicosanoids 5, 2 (1992): 87-97. [PubMed Link] |
Platz, M.; Admasu, AS ; Kwiatkowski, S.; Crocker, PJ ; Imai, N.; Watt, DS "Photolysis of 3-aryl-3-(trifluoromethyl)diazirines: a caveat regarding their use in photoaffinity probes." Bioconjugate chemistry 2, 5 (1991): 337-41. [PubMed Link] |
Leyva, E.; Chang, DH ; Platz, MS ; Watt, DS ; Crocker, PJ ; Kawada, K. "The photochemistry of iodo, methyl and thiomethyl substituted aryl azides in toluene solution and frozen polycrystals." Photochemistry and photobiology 54, 3 (1991): 329-33. [PubMed Link] | [ Full text ]
Kawada, K.; Pralong, E.; Vesin, MF ; Fitz, TA ; Watt, DS "Prostaglandin photoaffinity probes: synthesis and binding affinity of an azide-substituted 17-phenyl PGE2 prostaglandin." Eicosanoids 4, 1 (1991): 57-60. [PubMed Link] |
Chavan, AJ ; Kim, H.; Haley, BE ; Watt, DS "A photoactive phosphonamide derivative of GTP for the identification of the GTP-binding domain in beta-tubulin." Bioconjugate chemistry 1, 5 (1990): 337-44. [PubMed Link] |
Liu, YX ; Contois, DF ; Watt, DS ; Walden TL, Jr ; Fitz, TA "Interaction of leukotriene C4 and Chinese hamster lung fibroblasts (V79A03 cells). 2. Subcellular distribution of binding and unlikely role of glutathione-S-transferase." Prostaglandins 40, 4 (1990): 431-43. [PubMed Link] |
Fitz, TA ; Contois, DF ; Liu, YX ; Watt, DS ; Walden TL, Jr "Interaction of leukotriene C4 and Chinese hamster lung fibroblasts (V79A03 cells). 1. Characterization of binding." Prostaglandins 40, 4 (1990): 417-29. [PubMed Link] |
Crocker, PJ ; Imai, N.; Rajagopalan, K.; Boggess, MA ; Kwiatkowski, S.; Dwyer, LD ; Vanaman, TC ; Watt, DS "Heterobifunctional cross-linking agents incorporating perfluorinated aryl azides." Bioconjugate chemistry 1, 6 (1990): 419-24. [PubMed Link] |
Imai, N.; Dwyer, LD ; Kometani, T.; Ji, T.; Vanaman, TC ; Watt, DS "Photoaffinity heterobifunctional cross-linking reagents based on azide-substituted salicylates." Bioconjugate chemistry 1, 2 (1990): 144-8. [PubMed Link] |
Imai, N.; Kometani, T.; Crocker, PJ ; Bowdan, JB ; Demir, A.; Dwyer, LD ; Mann, DM ; Vanaman, TC ; Watt, DS "Photoaffinity heterobifunctional cross-linking reagents based on N-(azidobenzoyl)tyrosines." Bioconjugate chemistry 1, 2 (1990): 138-43. [PubMed Link] |
Chavan, AJ ; Rychlik, W.; Blaas, D.; Kuechler, E.; Watt, DS ; Rhoads, RE "Phenyl azide substituted and benzophenone-substituted phosphonamides of 7-methylguanosine 5'-triphosphate as photoaffinity probes for protein synthesis initiation factor eIF-4E and a proteolytic fragment containing the cap-binding site." Biochemistry 29, 23 (1990): 5521-9. [PubMed Link] |
Moldowan, JM ; Fago, FJ ; Lee, CY ; Jacobson, SR ; Watt, DS ; Slougui, NE ; Jeganathan, A.; Young, DC "Sedimentary 12-n-Propylcholestanes, Molecular Fossils Diagnostic of Marine Algae." Science (New York, N.Y.) 247, 4940 (1990): 309-12. [PubMed Link] | [ Full text ]
Balapure, AK ; Caicedo, IC ; Kawada, K.; Watt, DS ; Rexroad CE, Jr ; Fitz, TA "Multiple classes of prostaglandin F2 alpha binding sites in subpopulations of ovine luteal cells." Biology of reproduction 41, 3 (1989): 385-92. [PubMed Link] | [ Full text ]
Balapure, AK ; Rexroad CE, Jr ; Kawada, K.; Watt, DS ; Fitz, TA "Structural requirements for prostaglandin analog interaction with the ovine corpus luteum prostaglandin F2 alpha receptor. Implications for development of a photoaffinity probe." Biochemical pharmacology 38, 14 (1989): 2375-81. [PubMed Link] | [ Full text ]
Kawada, K.; Dolence, EK ; Morita, H.; Kometani, T.; Watt, DS ; Balapure, A.; Fitz, TA ; Orlicky, DJ ; Gerschenson, LE "Prostaglandin photoaffinity probes: synthesis and biological activity of azide-substituted 16-phenoxy- and 17-phenyl-PGF2 alpha prostaglandins." Journal of medicinal chemistry 32, 1 (1989): 256-64. [PubMed Link] |
Tobin, T.; Kwiatkowski, S.; Watt, DS ; Tai, HH ; Tai, CL ; Woods, WE ; Goodman, JP ; Taylor, DG ; Weckman, TJ ; Yang, JM "Immunoassay detection of drugs in racing horses. XI. ELISA and RIA detection of fentanyl, alfentanil, sufentanil and carfentanil in equine blood and urine." Research communications in chemical pathology and pharmacology 63, 1 (1989): 129-52. [PubMed Link] |
Ho, LT ; Nie, ZM ; Mende, TJ ; Richardson, S.; Chavan, A.; Kolaczkowska, E.; Watt, DS ; Haley, BE ; Ho, RJ "Modification of adenylate cyclase by photoaffinity analogs of forskolin." Second messengers and phosphoproteins 12, 5-6 (1988): 209-23. [PubMed Link] |
Kwiatkowski, S.; Sturma, L.; Dai, MR ; Tai, HH ; Watt, DS ; Tai, CL ; Woods, WE ; Weckman, TJ ; Yang, JM ; Wood, T. "Immunoassay detection of drugs in racing horses. VII. Detection of acepromazine in equine urine and blood by ELISA and PCFIA." Research communications in chemical pathology and pharmacology 61, 3 (1988): 391-412. [PubMed Link] |
Tobin, T.; Watt, DS ; Kwiatkowski, S.; Tai, HH ; Blake, JW ; McDonald, J ; Prange, CA ; Wie, S. "Non-isotopic immunoassay drug tests in racing horses: a review of their application to pre- and post-race testing, drug quantitation, and human drug testing." Research communications in chemical pathology and pharmacology 62, 3 (1988): 371-95. [PubMed Link] |
Schaeffer, TL ; Cantwell, SG ; Brown, JL ; Watt, DS ; Fall, RR "Microbial growth on hydrocarbons: terminal branching inhibits biodegradation." Applied and environmental microbiology 38, 4 (1979): 742-6. [PubMed Link] | [ Full text ]
Auel, RA ; Freerksen, RW ; Watt, DS "Various 18-substituted progesterones." Steroids 31, 3 (1978): 367-74. [PubMed Link] | [ Full text ]
Cantwell, SG ; Lau, EP ; Watt, DS ; Fall, RR "Biodegradation of acyclic isoprenoids by Pseudomonas species." Journal of bacteriology 135, 2 (1978): 324-33. [PubMed Link] | [ Full text ]
Freerksen, RW ; Pabst, WE ; Raggio, ML ; Sherman, SA ; Wroble, RR ; Watt, DS "Photolysis of alpha-peracetoxynitriles. 2.1 A comparison of two synthetic approaches to 18-cyano-20-ketosteroids." Journal of the American Chemical Society 99, 5 (1977): 1536-42. [PubMed Link] |
Raggio, ML ; Watt, DS "A synthesis of progesterone from dehydroepiandrosterone." The Journal of organic chemistry 41, 10 (1976): 1873-5. [PubMed Link] |
Griffin, JF ; Erman, MG ; Duax, WL ; Watt, DS ; Carey, FA "Configuration of 5-cholestene hydrochloride." The Journal of organic chemistry 40, 20 (1975): 2956-8. [PubMed Link] |
Spivack, J.; Watt, DS "Disposable OR packs." Hospitals 44, 13 (1970): 105-14. [PubMed Link] |
Hanlon, DP ; Watt, DS ; Westhead, EW "The interaction of divalent metal ions with tris buffer in dilute solution." Analytical biochemistry 16, 2 (1966): 225-33. [PubMed Link] | [ Full text ]

Postdoctoral Scholar Position University of Kentucky

A position is available on or before July 1, 2018, for a talented Ph.D. organic chemist with experience in organic synthesis and an understanding of biochemistry. The individual selected for this position will work in the general area of medicinal chemistry. Experience with various synthetic procedures, NMR techniques, and methods for the characterization of organic compounds is required. The position carries a competitive salary commensurate with experience. The position will involve an initial appointment at the University of Kentucky and if performance is satisfactory, will lead to a staff position in a company in mainland China.

A candidate should submit an electronic copy of his or her curriculum vita and three letters of recommendation to David Watt (dwatt@uky.edu), Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, KY 40536-0509. The review of applications will continue until the position is filled. The University of Kentucky is an EEO employer and encourages applications from minorities and women.

The initial worksite is a laboratory is located in the Biological and Pharmaceutical Building and is part of the Center for Pharmaceutical Research and Innovation at the University of Kentucky. Lexington is a city of approximately 300,000 in the Bluegrass region of Kentucky and is well known as a center of the horse racing industry. The University of Kentucky is ranked highly among public universities in the United States. For additional information, please see http://www.visitlex.com/