Haining Zhu, Ph.D.

Bio / Education: 

Ph.D., 2000, UCLA
B.S., 1994, Univ. Sci. & Tech. China

Research Description: 

We use an array of proteomic, biochemical and cell biology techniques to study neurodegenerative diseases, particularly amyotrophic lateral sclerosis (ALS, Lou Gehrig’s disease). We are interested in understanding protein degradation and aggregation, oxidative stress, mitochondrial dysfunction and axonal transport in ALS and other neurodegenerative diseases.

Developing innovative proteomic methodologies to enable quantitative analysis of proteins and protein modifications is another interest of the laboratory. Moreover, we have been collaborating to apply proteomic approaches to multiple biomedical fields, including prostate cancer, diabetes and environmental toxicology.

PubMed Publications: 

  • Elshahawi, S.I.;Ramelot, T.A.;Seetharaman, J.;Chen, J.;Singh, S.;Yang, Y.;Pederson, K.;Kharel, M.K.;Xiao, R.;Lew, S.;Yennamalli, R.M.;Miller, M.D.;Wang, F.;Tong, L.;Montelione, G.T.;Kennedy, M.A.;Bingman, C.A.;Zhu, H.;Phillips, G.N.;Thorson, J.S. "Structure-guided functional characterization of enediyne self-sacrifice resistance proteins CalU16 and CalU19." ACS chemical biology (2014): [PubMed Link] | [ Full text ]
  • Matveev, S.V.;Spielmann, H.P.;Metts, B.M.;Chen, J.;Onono, F.;Zhu, H.;Scheff, S.W.;Walker, L.C.;Levine H, 3rd "A distinct subfraction of Aβ is responsible for the high-affinity Pittsburgh compound B-binding site in Alzheimer's disease brain." Journal of neurochemistry (2014): [PubMed Link] | [ Full text ]
  • Shi, J.;Wang, Y.;Zeng, L.;Wu, Y.;Deng, J.;Zhang, Q.;Lin, Y.;Li, J.;Kang, T.;Tao, M.;Rusinova, E.;Zhang, G.;Wang, C.;Zhu, H.;Yao, J.;Zeng, Y.X.;Evers, B.M.;Zhou, M.M.;Zhou, B.P. "Disrupting the interaction of BRD4 with diacetylated Twist suppresses tumorigenesis in basal-like breast cancer." Cancer cell 25, 2 (2014): 210-25. [PubMed Link] | [ Full text ]
  • Dhar, S.K.;Zhang, J.;Gal, J.;Xu, Y.;Miao, L.;Lynn, B.C.;Zhu, H.;Kasarskis, E.J.;St Clair, D.K. "FUsed in sarcoma is a novel regulator of manganese superoxide dismutase gene transcription." Antioxidants & redox signaling 20, 10 (2014): 1550-66. [PubMed Link] | [ Full text ]
  • Jutras, B.L.;Chenail, A.M.;Carroll, D.W.;Miller, M.C.;Zhu, H.;Bowman, A.;Stevenson, B. "Bpur, the Lyme disease spirochete's PUR domain protein: identification as a transcriptional modulator and characterization of nucleic acid interactions." The Journal of biological chemistry 288, 36 (2013): 26220-34. [PubMed Link] | [ Full text ]
  • Wagner, J.M.;Evans, T.J.;Chen, J.;Zhu, H.;Houben, E.N.;Bitter, W.;Korotkov, K.V. "Understanding specificity of the mycosin proteases in ESX/type VII secretion by structural and functional analysis." Journal of structural biology 184, 2 (2013): 115-28. [PubMed Link] | [ Full text ]
  • Gal, J.;Chen, J.;Barnett, K.R.;Yang, L.;Brumley, E.;Zhu, H. "HDAC6 regulates mutant SOD1 aggregation through two SMIR motifs and tubulin acetylation." The Journal of biological chemistry 288, 21 (2013): 15035-45. [PubMed Link] | [ Full text ]
  • Li, X.;Zhou, Q.;Sunkara, M.;Kutys, M.L.;Wu, Z.;Rychahou, P.;Morris, A.J.;Zhu, H.;Evers, B.M.;Huang, C. "Ubiquitylation of phosphatidylinositol 4-phosphate 5-kinase type I γ by HECTD1 regulates focal adhesion dynamics and cell migration." Journal of cell science 126, Pt 12 (2013): 2617-28. [PubMed Link] | [ Full text ]
  • Liu, X.;Niu, C.;Ren, J.;Zhang, J.;Xie, X.;Zhu, H.;Feng, W.;Gong, W. "The RRM domain of human fused in sarcoma protein reveals a non-canonical nucleic acid binding site." Biochimica et biophysica acta 1832, 2 (2013): 375-85. [PubMed Link] | [ Full text ]
  • Pandya, R.S.;Zhu, H.;Li, W.;Bowser, R.;Friedlander, R.M.;Wang, X. "Therapeutic neuroprotective agents for amyotrophic lateral sclerosis." Cellular and molecular life sciences : CMLS 70, 24 (2013): 4729-45. [PubMed Link] | [ Full text ]
  • Convertini, P.;Zhang, J.;Grange, P.;Hayward, L.J.;Zhu, H.;Stamm, S. "Genome wide array analysis indicates that an amyotrophic lateral sclerosis mutation of FUS causes an early increase of CAMK2N2 in vitro." Biochimica et biophysica acta 1832, 8 (2013): 1129-35. [PubMed Link] | [ Full text ]
  • Zhao, J.;Chen, J.;Zhu, H.;Xiong, Y.L. "Mass spectrometric evidence of malonaldehyde and 4-hydroxynonenal adductions to radical-scavenging soy peptides." Journal of agricultural and food chemistry 60, 38 (2012): 9727-36. [PubMed Link] | [ Full text ]
  • Wang, G.;Pahari, P.;Kharel, M.K.;Chen, J.;Zhu, H.;Van Lanen, S.G.;Rohr, J. "Cooperation of two bifunctional enzymes in the biosynthesis and attachment of deoxysugars of the antitumor antibiotic mithramycin." Angewandte Chemie (International ed. in English) 51, 42 (2012): 10638-42. [PubMed Link] | [ Full text ]
  • Lu, W.;Chai, Q.;Zhong, M.;Yu, L.;Fang, J.;Wang, T.;Li, H.;Zhu, H.;Wei, Y. "Assembling of AcrB trimer in cell membrane." Journal of molecular biology 423, 1 (2012): 123-34. [PubMed Link] | [ Full text ]
  • Xia, R.;Liu, Y.;Yang, L.;Gal, J.;Zhu, H.;Jia, J. "Motor neuron apoptosis and neuromuscular junction perturbation are prominent features in a Drosophila model of Fus-mediated ALS." Molecular neurodegeneration 7, (2012): 10. [PubMed Link] | [ Full text ]
  • Tang, X.;Seyb, K.I.;Huang, M.;Schuman, E.R.;Shi, P.;Zhu, H.;Glicksman, M.A. "A high-throughput screening method for small-molecule inhibitors of the aberrant mutant SOD1 and dynein complex interaction." Journal of biomolecular screening 17, 3 (2012): 314-26. [PubMed Link] | [ Full text ]
  • Zhu, H.;Zhao, J.;Zhu, B.;Collazo, J.;Gal, J.;Shi, P.;Liu, L.;Ström, A.L.;Lu, X.;McCann, R.O.;Toborek, M.;Kyprianou, N. "EMMPRIN regulates cytoskeleton reorganization and cell adhesion in prostate cancer." The Prostate 72, 1 (2012): 72-81. [PubMed Link] | [ Full text ]
  • Martin, S.K.;Vaughan, T.B.;Atkinson, T.;Zhu, H.;Kyprianou, N. "Emerging biomarkers of prostate cancer (Review)." Oncology reports 28, 2 (2012): 409-17. [PubMed Link] | [ Full text ]
  • Niu, C.;Zhang, J.;Gao, F.;Yang, L.;Jia, M.;Zhu, H.;Gong, W. "FUS-NLS/Transportin 1 complex structure provides insights into the nuclear targeting mechanism of FUS and the implications in ALS." PloS one 7, 10 (2012): e47056. [PubMed Link] | [ Full text ]
  • Kuo, K.L.;Zhu, H.;McNamara, P.J.;Leggas, M. "Localization and functional characterization of the rat Oatp4c1 transporter in an in vitro cell system and rat tissues." PloS one 7, 6 (2012): e39641. [PubMed Link] | [ Full text ]
  • Gal, J.;Zhang, J.;Kwinter, D.M.;Zhai, J.;Jia, H.;Jia, J.;Zhu, H. "Nuclear localization sequence of FUS and induction of stress granules by ALS mutants." Neurobiology of aging 32, 12 (2011): 2323.e27-40. [PubMed Link] | [ Full text ]
  • Tang, X.;Tang, X.;Gal, J.;Kyprianou, N.;Zhu, H.;Tang, G. "Detection of microRNAs in prostate cancer cells by microRNA array." Methods in molecular biology (Clifton, N.J.) 732, (2011): 69-88. [PubMed Link] | [ Full text ]
  • Shi, P.;Ström, A.L.;Gal, J.;Zhu, H. "Effects of ALS-related SOD1 mutants on dynein- and KIF5-mediated retrograde and anterograde axonal transport." Biochimica et biophysica acta 1802, 9 (2010): 707-16. [PubMed Link] | [ Full text ]
  • Bargagna-Mohan, P.;Paranthan, R.R.;Hamza, A.;Dimova, N.;Trucchi, B.;Srinivasan, C.;Elliott, G.I.;Zhan, C.G.;Lau, D.L.;Zhu, H.;Kasahara, K.;Inagaki, M.;Cambi, F.;Mohan, R. "Withaferin A targets intermediate filaments glial fibrillary acidic protein and vimentin in a model of retinal gliosis." The Journal of biological chemistry 285, 10 (2010): 7657-69. [PubMed Link] | [ Full text ]
  • Zhu, B.;Zhai, J.;Zhu, H.;Kyprianou, N. "Prohibitin regulates TGF-beta induced apoptosis as a downstream effector of Smad-dependent and -independent signaling." The Prostate 70, 1 (2010): 17-26. [PubMed Link] | [ Full text ]
  • Shi, P.;Gal, J.;Kwinter, D.M.;Liu, X.;Zhu, H. "Mitochondrial dysfunction in amyotrophic lateral sclerosis." Biochimica et biophysica acta 1802, 1 (2010): 45-51. [PubMed Link] | [ Full text ]
  • Ma, Y.;Xiong, Y.L.;Zhai, J.;Zhu, H.;Dziubla, T. "Fractionation and evaluation of radical-scavenging peptides from in vitro digests of buckwheat protein." Food chemistry 118, 3 (2010): 582-588. [PubMed Link] |
  • Shi, P.;Wei, Y.;Zhang, J.;Gal, J.;Zhu, H. "Mitochondrial dysfunction is a converging point of multiple pathological pathways in amyotrophic lateral sclerosis." Journal of Alzheimer's disease : JAD 20 Suppl 2, (2010): S311-24. [PubMed Link] | [ Full text ]
  • Gal, J.;Ström, A.L.;Kwinter, D.M.;Kilty, R.;Zhang, J.;Shi, P.;Fu, W.;Wooten, M.W.;Zhu, H. "Sequestosome 1/p62 links familial ALS mutant SOD1 to LC3 via an ubiquitin-independent mechanism." Journal of neurochemistry 111, 4 (2009): 1062-73. [PubMed Link] | [ Full text ]
  • Zhai, J.;Ström, A.L.;Kilty, R.;Venkatakrishnan, P.;White, J.;Everson, W.V.;Smart, E.J.;Zhu, H. "Proteomic characterization of lipid raft proteins in amyotrophic lateral sclerosis mouse spinal cord." The FEBS journal 276, 12 (2009): 3308-23. [PubMed Link] | [ Full text ]
  • Liu, R.;Ström, A.L.;Zhai, J.;Gal, J.;Bao, S.;Gong, W.;Zhu, H. "Enzymatically inactive adenylate kinase 4 interacts with mitochondrial ADP/ATP translocase." The international journal of biochemistry & cell biology 41, 6 (2009): 1371-80. [PubMed Link] | [ Full text ]
  • Zhai, J.;Liu, X.;Huang, Z.;Zhu, H. "RABA (reductive alkylation by acetone): a novel stable isotope labeling approach for quantitative proteomics." Journal of the American Society for Mass Spectrometry 20, 7 (2009): 1366-77. [PubMed Link] | [ Full text ]
  • Liu, Y.;Chen, G.;Norton, N.;Liu, W.;Zhu, H.;Zhou, P.;Luan, M.;Yang, S.;Chen, X.;Carroll, L.;Williams, N.M.;O'Donovan, M.C.;Kirov, G.;Owen, M.J. "Whole genome association study in a homogenous population in Shandong peninsula of China reveals JARID2 as a susceptibility gene for schizophrenia." Journal of biomedicine & biotechnology 2009, (2009): 536918. [PubMed Link] | [ Full text ]
  • Zhu, H.;Taylor, J.W.;Bennett, D.A.;Younkin, S.G.;Estus, S. "Lack of association of hepatic lipase polymorphisms with late-onset Alzheimer's disease." Neurobiology of aging 29, 5 (2008): 793-4. [PubMed Link] | [ Full text ]
  • Govindaswami, M.;Brown, S.A.;Yu, J.;Zhu, H.;Bishop, P.D.;Kindy, M.S.;Oeltgen, P.R. "Delta 2-specific opioid receptor agonist and hibernating woodchuck plasma fraction provide ischemic neuroprotection." Academic emergency medicine : official journal of the Society for Academic Emergency Medicine 15, 3 (2008): 250-7. [PubMed Link] | [ Full text ]
  • Ström, A.L.;Gal, J.;Shi, P.;Kasarskis, E.J.;Hayward, L.J.;Zhu, H. "Retrograde axonal transport and motor neuron disease." Journal of neurochemistry 106, 2 (2008): 495-505. [PubMed Link] | [ Full text ]
  • Ramesh Babu, J.;Lamar Seibenhener, M.;Peng, J.;Strom, A.L.;Kemppainen, R.;Cox, N.;Zhu, H.;Wooten, M.C.;Diaz-Meco, M.T.;Moscat, J.;Wooten, M.W. "Genetic inactivation of p62 leads to accumulation of hyperphosphorylated tau and neurodegeneration." Journal of neurochemistry 106, 1 (2008): 107-20. [PubMed Link] | [ Full text ]
  • White, J.;Guerin, T.;Swanson, H.;Post, S.;Zhu, H.;Gong, M.;Liu, J.;Everson, W.V.;Li, X.A.;Graf, G.A.;Ballard, H.O.;Ross, S.A.;Smart, E.J. "Diabetic HDL-associated myristic acid inhibits acetylcholine-induced nitric oxide generation by preventing the association of endothelial nitric oxide synthase with calmodulin." American journal of physiology. Cell physiology 294, 1 (2008): C295-305. [PubMed Link] | [ Full text ]
  • Ström, A.L.;Shi, P.;Zhang, F.;Gal, J.;Kilty, R.;Hayward, L.J.;Zhu, H. "Interaction of amyotrophic lateral sclerosis (ALS)-related mutant copper-zinc superoxide dismutase with the dynein-dynactin complex contributes to inclusion formation." The Journal of biological chemistry 283, 33 (2008): 22795-805. [PubMed Link] | [ Full text ]
  • Tang, X.;Gal, J.;Zhuang, X.;Wang, W.;Zhu, H.;Tang, G. "A simple array platform for microRNA analysis and its application in mouse tissues." RNA (New York, N.Y.) 13, 10 (2007): 1803-22. [PubMed Link] | [ Full text ]
  • Hou, X.;Liu, R.;Ross, S.;Smart, E.J.;Zhu, H.;Gong, W. "Crystallographic studies of human MitoNEET." The Journal of biological chemistry 282, 46 (2007): 33242-6. [PubMed Link] | [ Full text ]
  • Zhang, F.;Ström, A.L.;Fukada, K.;Lee, S.;Hayward, L.J.;Zhu, H. "Interaction between familial amyotrophic lateral sclerosis (ALS)-linked SOD1 mutants and the dynein complex." The Journal of biological chemistry 282, 22 (2007): 16691-9. [PubMed Link] | [ Full text ]
  • Zhu, H.;Tucker, H.M.;Grear, K.E.;Simpson, J.F.;Manning, A.K.;Cupples, L.A.;Estus, S. "A common polymorphism decreases low-density lipoprotein receptor exon 12 splicing efficiency and associates with increased cholesterol." Human molecular genetics 16, 14 (2007): 1765-72. [PubMed Link] | [ Full text ]
  • Potter, S.Z.;Zhu, H.;Shaw, B.F.;Rodriguez, J.A.;Doucette, P.A.;Sohn, S.H.;Durazo, A.;Faull, K.F.;Gralla, E.B.;Nersissian, A.M.;Valentine, J.S. "Binding of a single zinc ion to one subunit of copper-zinc superoxide dismutase apoprotein substantially influences the structure and stability of the entire homodimeric protein." Journal of the American Chemical Society 129, 15 (2007): 4575-83. [PubMed Link] | [ Full text ]
  • Gal, J.;Ström, A.L.;Kilty, R.;Zhang, F.;Zhu, H. "p62 accumulates and enhances aggregate formation in model systems of familial amyotrophic lateral sclerosis." The Journal of biological chemistry 282, 15 (2007): 11068-77. [PubMed Link] | [ Full text ]
  • Zhu, B.;Fukada, K.;Zhu, H.;Kyprianou, N. "Prohibitin and cofilin are intracellular effectors of transforming growth factor beta signaling in human prostate cancer cells." Cancer research 66, 17 (2006): 8640-7. [PubMed Link] | [ Full text ]
  • Zhang, F.;Zhu, H. "Intracellular conformational alterations of mutant SOD1 and the implications for fALS-associated SOD1 mutant induced motor neuron cell death." Biochimica et biophysica acta 1760, 3 (2006): 404-14. [PubMed Link] | [ Full text ]
  • Dubal, D.B.;Rau, S.W.;Shughrue, P.J.;Zhu, H.;Yu, J.;Cashion, A.B.;Suzuki, S.;Gerhold, L.M.;Bottner, M.B.;Dubal, S.B.;Merchanthaler, I.;Kindy, M.S.;Wise, P.M. "Differential modulation of estrogen receptors (ERs) in ischemic brain injury: a role for ERalpha in estradiol-mediated protection against delayed cell death." Endocrinology 147, 6 (2006): 3076-84. [PubMed Link] | [ Full text ]
  • Berger, J.R.;Miller, C.S.;Mootoor, Y.;Avdiushko, S.A.;Kryscio, R.J.;Zhu, H. "JC virus detection in bodily fluids: clues to transmission." Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 43, 1 (2006): e9-12. [PubMed Link] | [ Full text ]
  • Miller, C.S.;Berger, J.R.;Mootoor, Y.;Avdiushko, S.A.;Zhu, H.;Kryscio, R.J. "High prevalence of multiple human herpesviruses in saliva from human immunodeficiency virus-infected persons in the era of highly active antiretroviral therapy." Journal of clinical microbiology 44, 7 (2006): 2409-15. [PubMed Link] | [ Full text ]
  • Gopalraj, R.K.;Zhu, H.;Kelly, J.F.;Mendiondo, M.;Pulliam, J.F.;Bennett, D.A.;Estus, S. "Genetic association of low density lipoprotein receptor and Alzheimer's disease." Neurobiology of aging 26, 1 (2005): 1-7. [PubMed Link] | [ Full text ]
  • Lu, X.;Zhu, H. "Tube-gel digestion: a novel proteomic approach for high throughput analysis of membrane proteins." Molecular & cellular proteomics : MCP 4, 12 (2005): 1948-58. [PubMed Link] | [ Full text ]
  • Fukada, K.;Zhang, F.;Vien, A.;Cashman, N.R.;Zhu, H. "Mitochondrial proteomic analysis of a cell line model of familial amyotrophic lateral sclerosis." Molecular & cellular proteomics : MCP 3, 12 (2004): 1211-23. [PubMed Link] | [ Full text ]
  • Zhu, H.;Hunter, T.C.;Pan, S.;Yau, P.M.;Bradbury, E.M.;Chen, X. "Residue-specific mass signatures for the efficient detection of protein modifications by mass spectrometry." Analytical chemistry 74, 7 (2002): 1687-94. [PubMed Link] |
  • Zhu, H.;Pan, S.;Gu, S.;Bradbury, E.M.;Chen, X. "Amino acid residue specific stable isotope labeling for quantitative proteomics." Rapid communications in mass spectrometry : RCM 16, 22 (2002): 2115-23. [PubMed Link] |
  • Dubal, D.B.;Zhu, H.;Yu, J.;Rau, S.W.;Shughrue, P.J.;Merchenthaler, I.;Kindy, M.S.;Wise, P.M. "Estrogen receptor alpha, not beta, is a critical link in estradiol-mediated protection against brain injury." Proceedings of the National Academy of Sciences of the United States of America 98, 4 (2001): 1952-7. [PubMed Link] | [ Full text ]
  • Zhu, H.;Yu, J.;Kindy, M.S. "Inhibition of amyloidosis using low-molecular-weight heparins." Molecular medicine (Cambridge, Mass.) 7, 8 (2001): 517-22. [PubMed Link] |
  • Xie, J.;Guo, Q.;Zhu, H.;Wooten, M.W.;Mattson, M.P. "Protein kinase C iota protects neural cells against apoptosis induced by amyloid beta-peptide." Brain research. Molecular brain research 82, 1-2 (2000): 107-13. [PubMed Link] | [ Full text ]
  • Keller, J.N.;Huang, F.F.;Zhu, H.;Yu, J.;Ho, Y.S.;Kindy, T.S. "Oxidative stress-associated impairment of proteasome activity during ischemia-reperfusion injury." Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 20, 10 (2000): 1467-73. [PubMed Link] | [ Full text ]
  • Mattson, M.P.;Zhu, H.;Yu, J.;Kindy, M.S. "Presenilin-1 mutation increases neuronal vulnerability to focal ischemia in vivo and to hypoxia and glucose deprivation in cell culture: involvement of perturbed calcium homeostasis." The Journal of neuroscience : the official journal of the Society for Neuroscience 20, 4 (2000): 1358-64. [PubMed Link] | [ Full text ]
  • Zhu, H.;Guo, Q.;Mattson, M.P. "Dietary restriction protects hippocampal neurons against the death-promoting action of a presenilin-1 mutation." Brain research 842, 1 (1999): 224-9. [PubMed Link] | [ Full text ]
  • Kindy, M.S.;Yu, J.;Guo, J.T.;Zhu, H. "Apolipoprotein Serum Amyloid A in Alzheimer's Disease." Journal of Alzheimer's disease : JAD 1, 3 (1999): 155-167. [PubMed Link] | [ Full text ]
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