Luke H. Bradley | Molecular & Cellular Biochemistry

Luke H. Bradley

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lhbradley@uky.edu
859-323-1826
MN 214 Medical Sciences Bldg
Lab webpage (offsite) https://lhbradley1.wixsite.com/bradleylab
UK START Program (offsite) https://jabr293.wixsite.com/startprogram

Position(s):

Regular Faculty

Affiliation(s):

Neuroscience

Bio / Education:

BS, Chemistry/Biochemistry, Ohio University
Ph.D., Biochemistry, Ohio State University
Postdoctoral Training, Chemistry/Synthetic Biology, Princeton University

Research Description:

Peptide and protein scaffolds for biotherapeutic discovery and development
Research in the Bradley laboratory (https://lhbradley1.wixsite.com/bradleylab) utilizes approaches at the interface of chemistry, biochemistry, and neurobiology to discover and develop peptide and protein-based molecules as platforms for various medical, biotechnical, and biotherapeutic applications.

UK START Program
The UK START Program (https://jabr293.wixsite.com/startprogram) is an University-Community partnership that works towards the recruitment and retention of first generation and underrepresented populations in STEM by providing year-round research opportunities and professional development activities for high school students and teachers on the University of Kentucky Campus.

Selected Publications:

Fuqua JL, Littrell OM, Lundblad M, Turchan-Cholewo J, Abdelmoti LG, Galperin E, Bradley LH, Cass WA, Gash DM, Gerhardt GA. (2014) Dynamic Changes in the Dopamine Neuron Function after DNSP-11 Treatment: Effects in vivo and Increased ERK 1/2 Phosphorylation in vitro. Peptides 54: 1-8.

Hardy PA, Keeley D, Schorn G, Forman, E, Ai Y, Zhang Z, Bradley LH. (2013) Convection enhanced delivery of gadolinium-tagged polylysine. J Neuroscience Methods, 219: 169-175.

Littrell OM, Fuqua JF, Richardson AD, Turchan-Cholewo J, Hascup ER, Huettl P, Pomerleau F, Bradley LH, Gash DM, Gerhardt GA. (2013) A synthetic five amino acid GDNF propeptide increases dopamine neuron differentiation and neurochemical function. Neuropeptides, 47: 43-49.

Magnani R, Chaffin B, Dick E, Bricken ML, Houtz RL, Bradley LH. (2012) Utilization of a calmodulin lysine methyltransferase co-expression system for the generation of a combinatorial library of post-translationally modified proteins. Protein Expression & Purification, 86: 83-88.

Sexton T, Boock LJ, Rodgers DR, Bradley LH, Hersh LB. (2012) Active site mutations change the substrate cleavage specificity of neprilysin. PLoS ONE, 7(2): e32343.

Arai R, Kobayashi N, Kimura A, Sato T, Wang AF, Platt JM, Bradley LH, Hecht MH. (2012) Domain-swapped dimeric structure of a de novo 4-helix bundle protein, WA20. J Phys Chem B, 116: 6569-7032.

Bradley LH, Bricken ML, Randle C. (2011) Expression, purification, and characterization of proteins from high-quality combinatorial libraries of the mammalian calmodulin central linker. Protein Expression & Purification 75: 186-192.

Kelps KA, Turchan-Cholewo J, Hascup ER, Taylor TL, Gash DM, Gerhardt GA, Bradley LH. (2011) Evaluation of the physical and in vitro protective activity of three synthetic peptides derived from the pro- and mature GDNF sequence. Neuropeptides 45(3): 213-218.

Bradley LH, Fuqua J, Richardson A, Turchan-Cholewo J, Ai Y, Kelps KA, Glass J, He X, Zhang Z, Grondin R, Littrell OM, Gash DM, Gerhardt GA. (2010) Dopamine neuron stimulating actions of a GDNF propeptide. PLoS ONE 5(3): e9752.

Patel SC, Bradley LH, Jinadasa SP, Hecht MH. (2009) Cofactor Binding and Enzymatic Activity in an Unevolved Superfamily of De Novo Designed 4-Helix Bundle Proteins. Protein Science, 18:1388-1400.

Bradley LH, Thumfort PP, Hecht MH. (2006) De novo proteins from binary patterned combinatorial libraries. Methods Mol Biol 340: 53-69.

Bradley LH, Kleiner RE, Wang AF, Hecht MH, Wood DW. (2005) An Intein-Based Genetic Selection Enables Construction of a High-Quality Library of Binary Patterned De Novo Protein Sequences. Protein Engineering, Design & Selection 18: 201-207.

PubMed Publications:

Grondin, R.; Littrell, OM ; Zhang, Z.; Ai, Y.; Huettl, P.; Pomerleau, F.; Quintero, JE ; Andersen, AH ; Stenslik, MJ ; Bradley, LH ; Lemmon, J.; O'Neill, MJ ; Gash, DM ; Gerhardt, GA "GDNF revisited: A novel mammalian cell-derived variant form of GDNF increases dopamine turnover and improves brain biodistribution." Neuropharmacology 147, (2019): 28-36. [PubMed Link] | [ Full text ]
Stenslik, MJ ; Evans, A.; Pomerleau, F.; Weeks, R.; Huettl, P.; Foreman, E.; Turchan-Cholewo, J.; Andersen, A.; Cass, WA ; Zhang, Z.; Grondin, RC ; Gash, DM ; Gerhardt, GA ; Bradley, LH "Methodology and effects of repeated intranasal delivery of DNSP-11 in awake Rhesus macaques." Journal of neuroscience methods 303, (2018): 30-40. [PubMed Link] | [ Full text ]
Smit, R.; Bouwens, RJ ; Carniani, S.; Oesch, PA ; Labbé, I.; Illingworth, GD ; Werf, P.; Bradley, LD ; Gonzalez, V.; Hodge, JA ; Holwerda, BW ; Maiolino, R.; Zheng, W. "Rotation in C ii -emitting gas in two galaxies at a redshift of 6.8." Nature 553, 7687 (2018): 178-181. [PubMed Link] | [ Full text ]
Stenslik, MJ ; Potts, LF ; Sonne, JW ; Cass, WA ; Turchan-Cholewo, J.; Pomerleau, F.; Huettl, P.; Ai, Y.; Gash, DM ; Gerhardt, GA ; Bradley, LH "Methodology and effects of repeated intranasal delivery of DNSP-11 in a rat model of Parkinson's disease." Journal of neuroscience methods 251, (2015): 120-9. [PubMed Link] | [ Full text ]
Fuqua, JL ; Littrell, OM ; Lundblad, M.; Turchan-Cholewo, J.; Abdelmoti, LG ; Galperin, E.; Bradley, LH ; Cass, WA ; Gash, DM ; Gerhardt, GA "Dynamic changes in dopamine neuron function after DNSP-11 treatment: effects in vivo and increased ERK 1/2 phosphorylation in vitro." Peptides 54, (2014): 1-8. [PubMed Link] | [ Full text ]
Bradley, LH "High-quality combinatorial protein libraries using the binary patterning approach." Methods in molecular biology (Clifton, N.J.) 1216, (2014): 117-28. [PubMed Link] | [ Full text ]
Hardy, PA ; Keeley, D.; Schorn, G.; Forman, E.; Ai, Y.; Venugopalan, R.; Zhang, Z.; Bradley, LH "Convection enhanced delivery of different molecular weight tracers of gadolinium-tagged polylysine." Journal of neuroscience methods 219, 1 (2013): 169-75. [PubMed Link] | [ Full text ]
Littrell, OM ; Fuqua, JL ; Richardson, AD ; Turchan-Cholewo, J.; Hascup, ER ; Huettl, P.; Pomerleau, F.; Bradley, LH ; Gash, DM ; Gerhardt, GA "A synthetic five amino acid propeptide increases dopamine neuron differentiation and neurochemical function." Neuropeptides 47, 1 (2013): 43-9. [PubMed Link] | [ Full text ]
Arai, R.; Kobayashi, N.; Kimura, A.; Sato, T.; Matsuo, K.; Wang, AF ; Platt, JM ; Bradley, LH ; Hecht, MH "Domain-swapped dimeric structure of a stable and functional de novo four-helix bundle protein, WA20." The journal of physical chemistry. B 116, 23 (2012): 6789-97. [PubMed Link] | [ Full text ]
Magnani, R.; Chaffin, B.; Dick, E.; Bricken, ML ; Houtz, RL ; Bradley, LH "Utilization of a calmodulin lysine methyltransferase co-expression system for the generation of a combinatorial library of post-translationally modified proteins." Protein expression and purification 86, 2 (2012): 83-8. [PubMed Link] | [ Full text ]
Sexton, T.; Hitchcook, LJ ; Rodgers, DW ; Bradley, LH ; Hersh, LB "Active site mutations change the cleavage specificity of neprilysin." PloS one 7, 2 (2012): e32343. [PubMed Link] | [ Full text ]
Kelps, KA ; Turchan-Cholewo, J.; Hascup, ER ; Taylor, TL ; Gash, DM ; Gerhardt, GA ; Bradley, LH "Evaluation of the physical and in vitro protective activity of three synthetic peptides derived from the pro- and mature GDNF sequence." Neuropeptides 45, 3 (2011): 213-8. [PubMed Link] | [ Full text ]
Fisher, MA ; McKinley, KL ; Bradley, LH ; Viola, SR ; Hecht, MH "De novo designed proteins from a library of artificial sequences function in Escherichia coli and enable cell growth." PloS one 6, 1 (2011): e15364. [PubMed Link] | [ Full text ]
Bradley, LH ; Bricken, ML ; Randle, C. "Expression, purification, and characterization of proteins from high-quality combinatorial libraries of the mammalian calmodulin central linker." Protein expression and purification 75, 2 (2011): 186-91. [PubMed Link] | [ Full text ]
Bradley, LH ; Fuqua, J.; Richardson, A.; Turchan-Cholewo, J.; Ai, Y.; Kelps, KA ; Glass, JD ; He, X.; Zhang, Z.; Grondin, R.; Littrell, OM ; Huettl, P.; Pomerleau, F.; Gash, DM ; Gerhardt, GA "Dopamine neuron stimulating actions of a GDNF propeptide." PloS one 5, 3 (2010): e9752. [PubMed Link] | [ Full text ]
Patel, SC ; Bradley, LH ; Jinadasa, SP ; Hecht, MH "Cofactor binding and enzymatic activity in an unevolved superfamily of de novo designed 4-helix bundle proteins." Protein science : a publication of the Protein Society 18, 7 (2009): 1388-400. [PubMed Link] | [ Full text ]
Bradley, LH ; Wei, Y.; Thumfort, P.; Wurth, C.; Hecht, MH "Protein design by binary patterning of polar and nonpolar amino acids." Methods in molecular biology (Clifton, N.J.) 352, (2007): 155-66. [PubMed Link] | [ Full text ]
Bradley, LH ; Thumfort, PP ; Hecht, MH "De novo proteins from binary-patterned combinatorial libraries." Methods in molecular biology (Clifton, N.J.) 340, (2006): 53-69. [PubMed Link] | [ Full text ]
Bradley, LH ; Kleiner, RE ; Wang, AF ; Hecht, MH ; Wood, DW "An intein-based genetic selection allows the construction of a high-quality library of binary patterned de novo protein sequences." Protein engineering, design & selection : PEDS 18, 4 (2005): 201-7. [PubMed Link] | [ Full text ]
Hecht, MH ; Das, A.; Go, A.; Bradley, LH ; Wei, Y. "De novo proteins from designed combinatorial libraries." Protein science : a publication of the Protein Society 13, 7 (2004): 1711-23. [PubMed Link] | [ Full text ]
Bradley, LH ; Swenson, RP "Role of hydrogen bonding interactions to N(3)H of the flavin mononucleotide cofactor in the modulation of the redox potentials of the Clostridium beijerinckii flavodoxin." Biochemistry 40, 30 (2001): 8686-95. [PubMed Link] | [ Full text ]
Chang, F.; Bradley, LH ; Swenson, RP "Evaluation of the hydrogen bonding interactions and their effects on the oxidation-reduction potentials for the riboflavin complex of the Desulfovibrio vulgaris flavodoxin." Biochimica et biophysica acta 1504, 2-3 (2001): 319-28. [PubMed Link] | [ Full text ]
Bradley, LH ; Swenson, RP "Role of glutamate-59 hydrogen bonded to N(3)H of the flavin mononucleotide cofactor in the modulation of the redox potentials of the Clostridium beijerinckii flavodoxin. Glutamate-59 is not responsible for the pH dependency but contributes to the..." Biochemistry 38, 38 (1999): 12377-86. [PubMed Link] | [ Full text ]
Christoff, JJ ; Bradley, L.; Miller, DD ; Lei, L.; Rodriguez, F.; Fraundorfer, P.; Romstedt, K.; Shams, G.; Feller, DR "Synthesis and evaluation of trimetoquinol derivatives: novel thromboxane A2/prostaglandin H2 antagonists with diminished beta-adrenergic agonist activity." Journal of medicinal chemistry 40, 1 (1997): 85-91. [PubMed Link] | [ Full text ]