2014

KATY BREWER (BS Biology, University of Kentucky)

Fellowships

  • Philanthropic Educational Organization (P.E.O.) Scholar Award, 2017
  • Kentucky Opportunity Fellowship, 2015
  • Northern Kentucky/Greater Cincinnati UK Alumni Club Fellowship, 2015

Awards

  • International Conference on Brain Energy Metabolism Travel Award, 2018
  • UK College of Medicine Student Travel Award, 2017
  • Philanthropic Educational Organization (P.E.O.) Scholar Award, 2017
  • 1st Place Poster Presentation, Department of Biochemistry Spring Research Conference, 2017
  • Outstanding Poster Award, 34th Annual Bluegrass Society for Neuroscience (BGSFN) Spring Neuroscience Day, 2017
  • ASBMB Graduate/Postdoctoral Travel Award, 2015
  • University of Kentucky College of Medicine Research Incentive Award, 2013-present     
  • NIH Institutional Development Award (IDeA) Young Investigators Travel Award, 2012

Publications

  • Maria Adelaida Garcia-Gimeno, Pilar Natalia Rodilla-Ramirez, Rosa Viana, Carla Rubio-Villena, Pablo Sanchez-Martin, Xavier Salas-Puig, M. Kathryn Brewer, Matthew S. Gentryand Pascual Sanz. Phenotypic characterization of a new EPM2A mutation (N163D) present in a patient with slow progression of Lafora disease. Submitted.
  • M. Kathryn Brewer, Amanda R. Sherwood, Vikas V. Dukhande, and Matthew S. Gentry.  Function and action of the glucan phosphatase laforin.  In Encyclopedia of Signaling Molecules.  Springer.  In press.
  • Matthew S. Gentry, M. Kathryn Brewer, Craig W. Vander Kooi. Structural Biology of Glucan Phosphatases from Humans to Plants. Review. Current Opinion in Structural Biology 2016, 40:62-69.
  • Shane Emanuelle#, M. Kathryn Brewer#, David A. Meekins, Matthew S. Gentry. Unique binding platforms among the glucan phosphatases. Review. Cellular and Molecular Life Sciences 2016, 73(14):2765-78.

These authors contributed equally to this work:

  • Madushi Raththagala, M. Kathryn Brewer, Matthew W. Parker, Amanda R. Sherwood, Brian K. Wong, Simon Hsu, Travis M. Bridges, Bradley C. Paasch, Lance M. Hellman, Satrio Husodo, David A. Meekins, Adam O. Taylor, Benjamin D. Turner, Kyle D. Auger, Vikas V. Dukhande, Srinivas Chakravarthy, Pascual Sanz, Virgil V. Woods Jr., Sheng Li, Craig W. Vander Kooi, and Matthew S. Gentry. Structural Mechanism of Laforin Function in Glycogen Dephosphorylation and Lafora Disease. Molecular Cell 2015, 57(2):261-72.
    • Ranjana Singh, M. Kathryn Brewer, Charles B. Mashburn, Dingyan Lou, Vimala Bondada, Brantley Graham, James W. Geddes. Calpain 5 is highly expressed in the CNS, carries dual nuclear localization signals, and is associated with nuclear Promyelocytic Leukemia Protein bodies. Journal of Biological Chemistry 2014, 289(28):19383-94.
    • M. Kathryn Brewer, Satrio Husodo, Vikas V. Dukhande, Mary Beth Johnson, Matthew S. Gentry. Expression, purification and characterization of soluble red rooster laforin as a fusion protein in Escherichia coli. BMC Biochemistry 2014, 15:8.

Presentations

  • Oral Presentation: “Lafora body degradation by a therapeutic enzyme for the treatment of Lafora Epilepsy.” 3rd International Lafora Disease Workshop; Barcelona, Spain, 2017       
  • Oral Presentation: “Lafora disease: a link between energy metabolism and epilepsy.”Kentucky Neuroscience Institute, Neurology Grand Rounds; Lexington, KY, 2016
    • Oral Presentation: “Mutation-specific mechanisms in Lafora disease.” 2nd International Lafora Disease Workshop; La Jolla, CA, 2016
    • Oral Presentation: “Mutation-specific mechanisms in Lafora disease.” University of Kentucky Department of Biochemistry Spring Research Conference; Lexington, KY         2016
    • Oral Presentation:“The Role of Laforin in Site-Specific Glycogen Dephosphorylation and Lafora Disease.” 4th Annual Bluegrass Biophysics Symposium; Lexington, KY 2015
    • Poster Presentation: “Treatment of Lafora Epilepsy by a therapeutic enzyme that degrades Lafora bodies.” 13th International Conference on Brain Energy Metabolism; Valdivia, Chile, 2018 
    • Poster Presentation: “Lafora body degradation by a therapeutic enzyme for the treatment of Lafora Epilepsy.” 32nd International Epilepsy Congress; Barcelona, Spain, 2017
    • Poster Presentation: “Lafora body degradation by a therapeutic enzyme for the treatment of Lafora Epilepsy.” 3rd International Lafora Disease Workshop; Barcelona, Spain, 2017
    • Poster Presentation: “Lafora body degradation by a therapeutic enzyme for the treatment of Lafora Epilepsy.” Department of Biochemistry Spring Research Conference; Lexington, KY, 2017
    • Poster Presentation: “Mutation-specific mechanisms in Lafora disease.” 6th Annual Bluegrass Biophysics Symposium; Lexington, KY, 2017
    • Poster Presentation: “Lafora body degradation by a therapeutic enzyme for the treatment of Lafora Epilepsy.” 34th Annual Bluegrass Society for Neuroscience (BGSFN) Spring Neuroscience Day; Lexington, KY, 2017                                           
    • Poster Presentation: “Mutation-specific mechanisms in Lafora disease.”  2nd International Lafora Disease Workshop; La Jolla, CA, 2016
      • Poster Presentation: “Mutation-specific mechanisms in Lafora disease.” University of Kentucky 5th Annual Epilepsy Symposium; Lexington, KY, 2016
      • Poster Presentation: “Defining the Tetramodular Dimer Structure of Laforin in Glycogen Metabolism and Lafora Disease.” 4th Annual Bluegrass Biophysics Symposium; Lexington, KY, 2015
      • Poster Presentation: “Defining the Tetramodular Dimer Structure of Laforin in Glycogen Metabolism and Lafora Disease.” ASBMB Annual Meeting; Boston, MA, 2015
      • Poster Presentation: “Calpain 5 is an atypical calpain highly expressed in the CNS, possesses dual nuclear localization signals, and is associated with Promyelocytic Leukemia nuclear bodies” 30th Annual BGSFN Spring Neuroscience Day; Lexington, KY, 2014
JOSHUA MITCHELL (BS Chemistry, University of Louisville)

Awards and Honors

  • ‘Honors’ in several medical school classes: Microscopic Anatomy, Human Embryology, Neurosciences, Genetics and Molecular Medicine, Clinical Neurosciences, Cancer Immunology, Special Topics: Biochemistry Research. Honors are awarded to students in the 90th percentile or greater and to students with over a 90% in the class (2012-14).
  • Course representative for Genetics and Molecular Medicine during medical school. As course representative, I acted as a liaison between the student body and the course director and professors. This included working out scheduling conflicts and disputing questions on exams (Spring 2013). 
  • 2nd Place Cancer Research Presentation Award Undergraduate Student Category for the “Computational Tools for the Identification of Detectable Uncharacterized Derivatized Metabolites within the Context of Known Metabolites” poster presented at Research Louisville (2012).
  • Recipient of the Hypercube Award, given to the graduating senior in the chemistry department with the highest GPA (2012).
  • Member of the three person team that won “Outstanding Winner”, one of the five teams with this rating internationally for the problem in discrete mathematics, in the 2012 Mathematical Competition in Modeling (MCM) Problem B for our paper “C.A.R.S.: Cellular Automaton Rafting Simulation”. The MCM is an annual, international mathematical modeling competition with over 3500 participating teams. My job on the project focused on writing the computer code for our model within the 72-hour deadline. This model was implemented in Perl and used multi-processing (2012).
  • Member of the team that received the Society of Industrial and Applied Mathematics Prize for the before mentioned “C.A.R.S.: Cellular Automaton Rafting Simulation” paper. The SIAM prize is awarded annually to one outstanding MCM team (2012).
  • Participant in the R25 NIH/NCI Cancer Education Program (R25-CA134283) under the direction of Dr.  David Hein. This program included education in research ethics and scientific writing (2012).
  • Grawemeyer Scholar at the University of Louisville (2008-2012).
  • Participant in the Guaranteed Entrance to Medical School (GEMS) program. This program takes up to ten graduating seniors each year and allows participants to shadow in a variety of clinical settings during undergrad (2008-2012).

Poster Presentations

  •          Joshua Mitchell and Hunter N.B. Moseley (2014) “Development of Large-Scale Metabolite Identification Methods for Metabolomics”. Poster presented at Bluegrass Biophysics Symposium, University of Kentucky, Lexington, Ky.
  •          Joshua Mitchell and Hunter N.B. Moseley (2014) “Development of Large-Scale Metabolite Identification Methods for Metabolomics”. Poster presented at Markey Cancer Research Day, University of Kentucky, Lexington, Ky.
  •          Joshua Mitchell and Hunter N.B. Moseley (2013) “Computational Tools for the Identification of Detectable Uncharacterized Derivatized Metabolites within the Context of Known Metabolites”. Poster presented at James Graham Brown Cancer Center 11th Annual Retreat, Louisville, Ky.­­
  •        Joshua Mitchell and Hunter N.B. Moseley (2013) “Computational Tools for the Identification of Detectable Uncharacterized Derivatized Metabolites within the Context of Known Metabolites”. Poster presented at UT-ORNL-KBRIN Bioinformatics Summit, Buchanan, TN.
  •        Joshua Mitchell and Hunter N.B. Moseley (2012) “Computational Tools for the Identification of Detectable Uncharacterized Derivatized Metabolites within the Context of Known Metabolites”. Poster presented at Research Louisville, University of Louisville, Louisville, Kentucky.
  •          Joshua Mitchell, James Jones and Suraj Kannan (2012) “C.A.R.S.: Cellular Automaton Rafting Simulation”. Paper presented at the Society of Industrial and Applied Mathematics Annual Meeting, Minneapolis, Minnesota.
  •          Joshua Mitchell and Hunter N.B. Moseley (2012) “Developing Computational Tools for Metabolite Identification in Ultra-High Resolution Mass Spectrometry Data”. Poster presented at University of Louisville Undergraduate Research Symposium, Louisville, Kentucky.
  •          Joshua Mitchell and Hunter N.B. Moseley (2012) “Developing Computational Tools for Metabolite Identification in Ultra-High Resolution Mass Spectrometry Data”. Poster presented at UT-ORNL-KBRIN Bioinformatics Summit, Louisville, Kentucky.
  •          Joshua Mitchell, Rima R. Patel, Rodney Folz Jr, Andrew McCullam and Hunter N.B. Moseley (2011) “Developing Computational Tools for Molecular Comparison and Metabolic Placement of Detectable Uncharacterized Metabolites”. James Graham Brown Cancer Center 10th Annual Retreat, Louisville, Kentucky.
  •          Joshua Mitchell, Rima R. Patel and Hunter N.B. Moseley (2011) “Developing Computational Tools for Metabolite Molecular Comparison and Search”. Poster presented at Posters-at-the-Capitol in Frankfort, Ky.

Publications

  •  Jones, J., Kannan, S., and Mitchell, J. (2013). Dynamic scheduling of white water rafting. Harvard College Mathematics Review 6, 96-112.
  • Mitchell, J.M., Fan, T.W., Lane, A.N., and Moseley, H.N. (2014). Development and in silico evaluation of large-scale metabolite identification methods using functional group detection for metabolomics. Frontiers in genetics 5, 237.

 

PAYTON STEVENS (BS Biology, University of Kentucky)

Fellowships

  • NIH/NCI F31 April 2015-January 2018

Publications

  • Stevens PD, Wen YA, Xiong X, Zayseva YY, Li A, Wang C, Farmer T, Inagaki M, Marchetto S, Borg JP, Gao T. Erbin suppresses colorectal cancer progression by antagonizing KSR1-mediated RAS/RAF signaling. Submitted to Cancer Research. November 2017.
  • Jafari N, Drury J, Morris AJ, Onono FO, Stevens PD, Gao T, Lee EY, Weiss HL, Evers BM, Zaytseva YY. De novo fatty acid synthesis driven sphingolipid metabolism promotes metastatic potential of colorectal cancer. Submitted to Cancer Research. November 2017.
  • Smith AJ, Wen YA, Stevens PD, Liu J, Wang C, Gao T. PHLPP negatively regulates cell motility through inhibition of Akt activity and integrin expression in pancreatic cancer cells. Oncotarget. 2016. doi: 10.18632/oncotarget.6848. PubMed PMID: 26760962
  • Li X, Stevens PD, Liu J, Yang H, Wang W, Wang C, Zeng Z, Schmidt MD, Yang M, Lee EY, Gao T. PHLPP is a negative regulator of RAF1, which reduces colorectal cancer cell motility and prevents tumor progression in mice. Gastroenterology. 2014;146(5):1301-12 e1-10. doi: 10.1053/j.gastro.2014.02.003. PubMed PMID: 24530606
  • Wen YA, Stevens PD, Gasser ML, Andrei R, Gao T. Downregulation of PHLPP expression contributes to hypoxia-induced resistance to chemotherapy in colon cancer cells. Molecular and cellular biology. 2013;33(22):4594-605. doi: 10.1128/MCB.00695-13. PubMed PMID: 24061475
  • Liu J, Stevens PD, Eshleman NE, Gao T. Protein phosphatase PPM1G regulates protein translation and cell growth by dephosphorylating 4E binding protein 1 (4E-BP1). The Journal of biological chemistry. 2013;288(32):23225-33. doi: 10.1074/jbc.M113.492371. PubMed PMID: 23814053
  • Li X, Stevens PD, Yang H, Gulhati P, Wang W, Evers BM, Gao T. The deubiquitination enzyme USP46 functions as a tumor suppressor by controlling PHLPP-dependent attenuation of Akt signaling in colon cancer. Oncogene. 2013;32(4):471-8. doi: 10.1038/onc.2012.66. PubMed PMID: 22391563
  • Gulhati P, Zaytseva YY, Valentino JD, Stevens PD, Kim JT, Sasazuki T, Shirasawa S, Lee EY, Weiss HL, Dong J, Gao T, Evers BM. Sorafenib enhances the therapeutic efficacy of rapamycin in colorectal cancers harboring oncogenic KRAS and PIK3CA. Carcinogenesis. 2012;33(9):1782-90. doi: 10.1093/carcin/bgs203. PubMed PMID: 22696593
  • Liu J, Stevens PD, Li X, Schmidt MD, Gao T. PHLPP-mediated dephosphorylation of S6K1 inhibits protein translation and cell growth. Molecular and cellular biology. 2011;31(24):4917-27. doi: 10.1128/MCB.05799-11. PubMed PMID: 21986499
  • Liu J, Stevens PD, Gao T. mTOR-dependent regulation of PHLPP expression controls the rapamycin sensitivity in cancer cells. The Journal of biological chemistry. 2011;286(8):6510-20. doi: 10.1074/jbc.M110.183087. PubMed PMID: 21177869
  • Gulhati P, Bowen KA, Liu J, Stevens PD, Rychahou PG, Chen M, Lee EY, Weiss HL, O'Connor KL, Gao T, Evers BM. mTORC1 and mTORC2 regulate EMT, motility, and metastasis of colorectal cancer via RhoA and Rac1 signaling pathways. Cancer research. 2011;71(9):3246-56. doi: 10.1158/0008-5472.CAN-10-4058. PubMed PMID: 21430067
  •  Larson Y, Liu J, Stevens PD, Li X, Li J, Evers BM, Gao T. Tuberous sclerosis complex 2 (TSC2) regulates cell migration and polarity through activation of CDC42 and RAC1. The Journal of biological chemistry. 2010;285(32):24987-98. doi: 10.1074/jbc.M109.096917. PubMed PMID: 20530489

 

Presentations

  • Oral Presentation: “Erbin Inhibits Colon Cancer Progression by Antagonizing KSR1-Facilitated RAS/RAF Signaling” Selected Student Speaker, Markey Cancer Center Annual Research Day, University of Kentucky, 2017”
  • Oral Presentation: “My Path to a Research Career” Selected Speaker, Biomedical Career Seminar, Markey Cancer Center Career Training in Oncology Program, University of Kentucky, 2016
  • Oral Presentation: “The Role of Erin in Colon Cancer” Student Seminar Presentation, Department of Molecular and Cellular Biochemistry, University of Kentucky, 2016
  • Oral Presentation: “The Tumor Suppressor Function of Erbin in Colon Cancer” Selected Student Speaker, Department of Molecular and Cellular Biochemistry, Annual Retreat, University of Kentucky, 2016
  • Oral Presentation:  “High-Fat Diet Induced PPAR-delta Activity Increases Stemness” Student Seminar Presentation, Department of Molecular and Cellular Biochemistry, University of Kentucky , 2016
  • Oral Presentation: “Erbin-mediated Regulation of Colon Cancer” Student Seminar Presentation, Department of Molecular and Cellular Biochemistry, University of Kentucky, 2015