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Sidney W. Whiteheart, PhD
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whitehe@uky.edu
859-257-4882
BBSRB 351, 741 S Limestone St, Lexington, KY 40536-0509
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Position(s): 
Professor
Affiliation(s): 
Molecular & Cellular Biochemistry
Cardiovascular Research Center
MD/PhD Program
Other Affiliation(s): 
MD/PhD Program Internal Advisory Committee
MD/PhD Program Mentor
CVRC - Affiliated Faculty
Bio / Education: 

B.S. Emory University
Ph.D. The Johns Hopkins University School of Medicine

Dr. Sidney (Wally) Whiteheart, professor in the department of molecular and cellular biochemistry, joined the College of Medicine in 1994. He has been an active member of the college community, serving as chair of the faculty council, a member of the strategic plan executive oversight committee, and interim chair of his department. He has mentored more than 50 trainees and is an active researcher focusing on platelet function and cell biology, with two R01 grants and a VA Merit grant. As the director, he will provide oversight for the program; ensure appropriate infrastructure and integration with the Integrated Biomedical Sciences program, oversee the graduate mentor/advisory committees and the co-directors, and cultivate and maintain an open-door culture for the MD/PhD students.

Research Description: 

∂  Molecular Mechanisms of Platelet Secretion
∂ Role of ADP Ribosylation Factor 6 (Arf6) in Platelet Activation
∂ Structure/Function Analysis of the General Fusion Protein: NSF


Platelets are essential to maintaining a pressurized vasculature. The platelet's role is to sense vascular damage and to promote clot formation and tissue repair. Secretion from their three granular stores is essential to hemostasis and our goal is to understand the mechanisms of this secretory process. In the resting platelet, hemostatic and tissue repair-inducing components are stored in three types of membrane bound granules. Upon stimulation, these granules are targeted to specific sites on the platelet's plasma membrane where the granule membranes fuse with the plasma membrane thus releasing the granule contents. Our studies are designed to determine what platelet proteins are involved in the steps leading to secretion. We are specifically interested in how the activities of these proteins are controlled by the signaling cascades that are initiated by external events such as vascular damage.

As part of these studies, we have also focused on a Ras-related ,small GTP binding protein called ADP Ribosylation Factors 6 (Arf6).  In our groundbreaking studies, we have shown that Arf6 is critical for the platelet cytoskeletal rearrangements needed for aggregation and clot formation.  This work is particularly exciting since it places Arf6 as a central regulator of many of the steps that occur in activated platelets.

NSF: All membrane trafficking events are mediated by integral membrane proteins (SNAREs) that span the two fusing bilayers and promote membrane fusion. Once fusion is accomplished, the complex formed by the spent SNAREs must be disassembled for recycling. Our group focuses on the enzyme that disassembles all cellular SNARE complexes and is thus essential to all membrane trafficking events. The N-ethylmaleimide Sensitive Fusion protein (NSF) is a homo-hexamer whose subunits contain three domains (an amino and two ATP binding domains). It is our hypothesis that each domain of NSF is responsible for a distinct aspect of the SNARE recycling process. Our laboratory uses structure-based mutagenesis together with a battery of assays to determine what parts of the NSF hexamer are responsible for the various processes required to disassemble SNARE complexes.

PubMed Publications: 
  • Huang, P.; Zhao, C.; Chen, C.; Whiteheart, SW ; Liu-Chen, LY "Does GEC1 Enhance Expression and Forward Trafficking of the Kappa Opioid Receptor (KOR) via Its Ability to Interact with NSF Directly?" Handbook of experimental pharmacology (2021): [PubMed Link] | [ Full text ]
  • Pokrovskaya, ID ; Tobin, M ; Desai, R.; Joshi, S.; Kamykowski, JA ; Zhang, G.; Aronova, MA ; Whiteheart, SW ; Leapman, RD ; Storrie, B. "Canalicular system reorganization during mouse platelet activation as revealed by 3D ultrastructural analysis." Platelets 32, 1 (2021): 97-104. [PubMed Link] | [ Full text ]
  • Chanzu, H ; Lykins, J ; Wigna-Kumar, S ; Joshi, S.; Pokrovskaya, I.; Storrie, B.; Pejler, G.; Wood, JP ; Whiteheart, SW "Platelet α-Granule Cargo Packaging and Release are Affected by the Luminal Proteoglycan, Serglycin." Journal of thrombosis and haemostasis : JTH (2021): [PubMed Link] | [ Full text ]
  • Xiang, B.; Zhang, G.; Zhang, Y.; Wu, C.; Joshi, S.; Morris, AJ ; Ware, J.; Smyth, SS ; Whiteheart, SW ; Li, Z. "Calcium Ion Chelation Preserves Platelet Function During Cold Storage." Arteriosclerosis, thrombosis, and vascular biology 41, 1 (2021): 234-249. [PubMed Link] | [ Full text ]
  • Gupta, S.; Konradt, C ; Corken, A ; Ware, J.; Nieswandt, B ; Di Paola, J ; Yu, M.; Wang, D.; Nieman, MT ; Whiteheart, SW ; Brass, LF "Hemostasis vs. homeostasis: Platelets are essential for preserving vascular barrier function in the absence of injury or inflammation." Proceedings of the National Academy of Sciences of the United States of America 117, 39 (2020): 24316-24325. [PubMed Link] | [ Full text ]
  • Banerjee, M.; Huang, Y.; Joshi, S.; Popa, GJ ; Mendenhall, MD ; Wang, QJ ; Garvy, BA ; Myint, T.; Whiteheart, SW "Platelets Endocytose Viral Particles and Are Activated via TLR (Toll-Like Receptor) Signaling." Arteriosclerosis, thrombosis, and vascular biology 40, 7 (2020): 1635-1650. [PubMed Link] | [ Full text ]
  • Butler, CR ; Boychuk, JA ; Pomerleau, F.; Alcala, R ; Huettl, P.; Ai, Y.; Jakobsson, J ; Whiteheart, SW ; Gerhardt, GA ; Smith, BN ; Slevin, JT "Modulation of epileptogenesis: A paradigm for the integration of enzyme-based microelectrode arrays and optogenetics." Epilepsy research 159, (2020): 106244. [PubMed Link] | [ Full text ]
  • Pokrovskaya, I.; Tobin, M ; Desai, R.; Aronova, MA ; Kamykowski, JA ; Zhang, G.; Joshi, S.; Whiteheart, SW ; Leapman, RD ; Storrie, B. "Structural analysis of resting mouse platelets by 3D-EM Reveals an unexpected variation in α-granule shape." Platelets (2020): 1-10. [PubMed Link] | [ Full text ]
  • Chow, KM ; Whiteheart, SW ; Smiley, JR ; Sharma, S.; Boaz, K ; Coleman, MJ ; Maynard, A ; Hersh, LB ; Vander Kooi, CW "Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies." Journal of visualized experiments : JoVE 143 (2019): [PubMed Link] | [ Full text ]
  • Adili, R.; Voigt, EM ; Bormann, JL ; Foss, KN ; Hurley, LJ ; Meyer, ES ; Veldman, AJ ; Mast, KA ; West, JL ; Whiteheart, SW ; Holinstat, M.; Larson, MK "In vivo modeling of docosahexaenoic acid and eicosapentaenoic acid-mediated inhibition of both platelet function and accumulation in arterial thrombi." Platelets 30, 2 (2019): 271-279. [PubMed Link] | [ Full text ]
  • Banerjee, M.; Huang, Y.; Ouseph, MM ; Joshi, S.; Pokrovskaya, I.; Storrie, B.; Zhang, J.; Whiteheart, SW ; Wang, QJ "Autophagy in Platelets." Methods in molecular biology (Clifton, N.J.) 1880, (2019): 511-528. [PubMed Link] | [ Full text ]
  • Joshi, S.; Banerjee, M.; Zhang, J.; Kesaraju, A ; Pokrovskaya, ID ; Storrie, B.; Whiteheart, SW "Alterations in platelet secretion differentially affect thrombosis and hemostasis." Blood advances 2, 17 (2018): 2187-2198. [PubMed Link] | [ Full text ]
  • Pokrovskaya, ID ; Joshi, S.; Tobin, M ; Desai, R.; Aronova, MA ; Kamykowski, JA ; Zhang, G.; Whiteheart, SW ; Leapman, RD ; Storrie, B. "SNARE-dependent membrane fusion initiates α-granule matrix decondensation in mouse platelets." Blood advances 2, 21 (2018): 2947-2958. [PubMed Link] | [ Full text ]
  • Zhang, J.; Huang, Y.; Chen, J.; Zhu, H.; Whiteheart, SW "Dynamic cycling of t-SNARE acylation regulates platelet exocytosis." The Journal of biological chemistry 293, 10 (2018): 3593-3606. [PubMed Link] | [ Full text ]
  • Whiteheart, SW "BEACHcombing for α-granules." Blood 131, 9 (2018): 949-950. [PubMed Link] | [ Full text ]
  • Banerjee, M.; Whiteheart, SW "The ins and outs of endocytic trafficking in platelet functions." Current opinion in hematology 24, 5 (2017): 467-474. [PubMed Link] | [ Full text ]
  • Whiteheart, SW "Fueling Platelets: Where Does the Glucose Come From?" Arteriosclerosis, thrombosis, and vascular biology 37, 9 (2017): 1592-1594. [PubMed Link] | [ Full text ]
  • Batten, SR ; Matveeva, EA ; Whiteheart, SW ; Vanaman, TC ; Gerhardt, GA ; Slevin, JT "Linking kindling to increased glutamate release in the dentate gyrus of the hippocampus through the STXBP5/tomosyn-1 gene." Brain and behavior 7, 9 (2017): e00795. [PubMed Link] | [ Full text ]
  • Joshi, S.; Whiteheart, SW "The nuts and bolts of the platelet release reaction." Platelets 28, 2 (2017): 129-137. [PubMed Link] | [ Full text ]
  • Storrie, B.; Whiteheart, SW "Editorial: Platelet Secretion." Platelets 28, 2 (2017): 107. [PubMed Link] | [ Full text ]
  • Banerjee, M.; Joshi, S.; Zhang, J.; Moncman, CL ; Yadav, S.; Bouchard, BA ; Storrie, B.; Whiteheart, SW "Cellubrevin/vesicle-associated membrane protein-3-mediated endocytosis and trafficking regulate platelet functions." Blood 130, 26 (2017): 2872-2883. [PubMed Link] | [ Full text ]
  • Eckly, A.; Rinckel, JY ; Proamer, F.; Ulas, N.; Joshi, S.; Whiteheart, SW ; Gachet, C. "Respective contributions of single and compound granule fusion to secretion by activated platelets." Blood 128, 21 (2016): 2538-2549. [PubMed Link] | [ Full text ]
  • Huang, Y.; Joshi, S.; Xiang, B.; Kanaho, Y.; Li, Z.; Bouchard, BA ; Moncman, CL ; Whiteheart, SW "Arf6 controls platelet spreading and clot retraction via integrin αIIbβ3 trafficking." Blood 127, 11 (2016): 1459-67. [PubMed Link] | [ Full text ]
  • Chicka, MC ; Ren, Q.; Richards, D.; Hellman, LM ; Zhang, J.; Fried, MG ; Whiteheart, SW "Role of Munc13-4 as a Ca2+-dependent tether during platelet secretion." The Biochemical journal 473, 5 (2016): 627-39. [PubMed Link] | [ Full text ]
  • Jones, MB ; Oswald, DM ; Joshi, S.; Whiteheart, SW ; Orlando, R.; Cobb, BA "B-cell-independent sialylation of IgG." Proceedings of the National Academy of Sciences of the United States of America 113, 26 (2016): 7207-12. [PubMed Link] | [ Full text ]
  • Banerjee, M.; Whiteheart, SW "How Does Protein Disulfide Isomerase Get Into a Thrombus?" Arteriosclerosis, thrombosis, and vascular biology 36, 6 (2016): 1056-7. [PubMed Link] | [ Full text ]
  • Ouseph, MM ; Huang, Y.; Banerjee, M.; Joshi, S.; MacDonald, L.; Zhong, Y.; Liu, H.; Li, X.; Xiang, B.; Zhang, G.; Komatsu, M.; Yue, Z.; Li, Z.; Storrie, B.; Whiteheart, SW ; Wang, QJ "Autophagy is induced upon platelet activation and is essential for hemostasis and thrombosis." Blood 126, 10 (2015): 1224-33. [PubMed Link] | [ Full text ]
  • Huang, Y.; Whiteheart, SW "Platelet secretion paves the way." Blood 126, 4 (2015): 433-4. [PubMed Link] | [ Full text ]
  • Xiang, B.; Zhang, G.; Ye, S.; Zhang, R.; Huang, C.; Liu, J.; Tao, M.; Ruan, C.; Smyth, SS ; Whiteheart, SW ; Li, Z. "Characterization of a Novel Integrin Binding Protein, VPS33B, Which Is Important for Platelet Activation and In Vivo Thrombosis and Hemostasis." Circulation 132, 24 (2015): 2334-44. [PubMed Link] | [ Full text ]
  • Ye, S.; Huang, Y.; Joshi, S.; Zhang, J.; Yang, F.; Zhang, G.; Smyth, SS ; Li, Z.; Takai, Y.; Whiteheart, SW "Platelet secretion and hemostasis require syntaxin-binding protein STXBP5." The Journal of clinical investigation 124, 10 (2014): 4517-28. [PubMed Link] | [ Full text ]
  • Jonnalagadda, D.; Sunkara, M.; Morris, AJ ; Whiteheart, SW "Granule-mediated release of sphingosine-1-phosphate by activated platelets." Biochimica et biophysica acta 1841, 11 (2014): 1581-9. [PubMed Link] | [ Full text ]
  • Nair-Gupta, P.; Baccarini, A.; Tung, N.; Seyffer, F.; Florey, O.; Huang, Y.; Banerjee, M.; Overholtzer, M.; Roche, PA ; Tampe, R.; Brown, BD ; Amsen, D.; Whiteheart, SW ; Blander, JM "TLR signals induce phagosomal MHC-I delivery from the endosomal recycling compartment to allow cross-presentation." Cell 158, 3 (2014): 506-21. [PubMed Link] | [ Full text ]
  • Whiteheart, SW "α-Granules at the BEACH." Blood 122, 19 (2013): 3247-8. [PubMed Link] | [ Full text ]
  • Karim, ZA ; Zhang, J.; Banerjee, M.; Chicka, MC ; Al Hawas, R.; Hamilton, TR ; Roche, PA ; Whiteheart, SW "IκB kinase phosphorylation of SNAP-23 controls platelet secretion." Blood 121, 22 (2013): 4567-74. [PubMed Link] | [ Full text ]
  • Xiang, B.; Zhang, G.; Guo, L.; Li, XA ; Morris, AJ ; Daugherty, A.; Whiteheart, SW ; Smyth, SS ; Li, Z. "Platelets protect from septic shock by inhibiting macrophage-dependent inflammation via the cyclooxygenase 1 signalling pathway." Nature communications 4, (2013): 2657. [PubMed Link] | [ Full text ]
  • Al Hawas, R.; Ren, Q.; Ye, S.; Karim, ZA ; Filipovich, AH ; Whiteheart, SW "Munc18b/STXBP2 is required for platelet secretion." Blood 120, 12 (2012): 2493-500. [PubMed Link] | [ Full text ]
  • Ye, S.; Karim, ZA ; Al Hawas, R.; Pessin, JE ; Filipovich, AH ; Whiteheart, SW "Syntaxin-11, but not syntaxin-2 or syntaxin-4, is required for platelet secretion." Blood 120, 12 (2012): 2484-92. [PubMed Link] | [ Full text ]
  • Xiang, B.; Zhang, G.; Stefanini, L.; Bergmeier, W.; Gartner, TK ; Whiteheart, SW ; Li, Z. "The Src family kinases and protein kinase C synergize to mediate Gq-dependent platelet activation." The Journal of biological chemistry 287, 49 (2012): 41277-87. [PubMed Link] | [ Full text ]
  • Matveeva, EA ; Price, DA ; Whiteheart, SW ; Vanaman, TC ; Gerhardt, GA ; Slevin, JT "Reduction of vesicle-associated membrane protein 2 expression leads to a kindling-resistant phenotype in a murine model of epilepsy." Neuroscience 202, (2012): 77-86. [PubMed Link] | [ Full text ]
  • Matveeva, EA ; Davis, VA ; Whiteheart, SW ; Vanaman, TC ; Gerhardt, GA ; Slevin, JT "Kindling-induced asymmetric accumulation of hippocampal 7S SNARE complexes correlates with enhanced glutamate release." Epilepsia 53, 1 (2012): 157-67. [PubMed Link] | [ Full text ]
  • Zhao, C.; Smith, EC ; Whiteheart, SW "Requirements for the catalytic cycle of the N-ethylmaleimide-Sensitive Factor (NSF)." Biochimica et biophysica acta 1823, 1 (2012): 159-71. [PubMed Link] | [ Full text ]
  • Moeller, A.; Zhao, C.; Fried, MG ; Wilson-Kubalek, EM ; Carragher, B.; Whiteheart, SW "Nucleotide-dependent conformational changes in the N-Ethylmaleimide Sensitive Factor (NSF) and their potential role in SNARE complex disassembly." Journal of structural biology 177, 2 (2012): 335-43. [PubMed Link] | [ Full text ]
  • Jonnalagadda, D.; Izu, LT ; Whiteheart, SW "Platelet secretion is kinetically heterogeneous in an agonist-responsive manner." Blood 120, 26 (2012): 5209-16. [PubMed Link] | [ Full text ]
  • Zhang, G.; Xiang, B.; Ye, S.; Chrzanowska-Wodnicka, M.; Morris, AJ ; Gartner, TK ; Whiteheart, SW ; White GC, 2nd ; Smyth, SS ; Li, Z. "Distinct roles for Rap1b protein in platelet secretion and integrin αIIbβ3 outside-in signaling." The Journal of biological chemistry 286, 45 (2011): 39466-77. [PubMed Link] | [ Full text ]
  • Hellman, LM ; Zhao, C.; Melikishvili, M.; Tao, X.; Hopper, JE ; Whiteheart, SW ; Fried, MG "Histidine-tag-directed chromophores for tracer analyses in the analytical ultracentrifuge." Methods (San Diego, Calif.) 54, 1 (2011): 31-8. [PubMed Link] | [ Full text ]
  • Whiteheart, SW "Platelet granules: surprise packages." Blood 118, 5 (2011): 1190-1. [PubMed Link] | [ Full text ]
  • Feng, W.; Madajka, M.; Kerr, BA ; Mahabeleshwar, GH ; Whiteheart, SW ; Byzova, TV "A novel role for platelet secretion in angiogenesis: mediating bone marrow-derived cell mobilization and homing." Blood 117, 14 (2011): 3893-902. [PubMed Link] | [ Full text ]
  • Zhao, C.; Matveeva, EA ; Ren, Q.; Whiteheart, SW "Dissecting the N-ethylmaleimide-sensitive factor: required elements of the N and D1 domains." The Journal of biological chemistry 285, 1 (2010): 761-72. [PubMed Link] | [ Full text ]
  • Kondkar, AA ; Bray, MS ; Leal, SM ; Nagalla, S.; Liu, DJ ; Jin, Y.; Dong, JF ; Ren, Q.; Whiteheart, SW ; Shaw, C.; Bray, PF "VAMP8/endobrevin is overexpressed in hyperreactive human platelets: suggested role for platelet microRNA." Journal of thrombosis and haemostasis : JTH 8, 2 (2010): 369-78. [PubMed Link] | [ Full text ]
  • Ren, Q.; Wimmer, C.; Chicka, MC ; Ye, S.; Ren, Y.; Hughson, FM ; Whiteheart, SW "Munc13-4 is a limiting factor in the pathway required for platelet granule release and hemostasis." Blood 116, 6 (2010): 869-77. [PubMed Link] | [ Full text ]
  • Zhao, C.; Hellman, LM ; Zhan, X.; Bowman, WS ; Whiteheart, SW ; Fried, MG "Hexahistidine-tag-specific optical probes for analyses of proteins and their interactions." Analytical biochemistry 399, 2 (2010): 237-45. [PubMed Link] | [ Full text ]
  • Choi, W.; Karim, ZA ; Whiteheart, SW "Protein expression in platelets from six species that differ in their open canalicular system." Platelets 21, 3 (2010): 167-75. [PubMed Link] | [ Full text ]
  • Graham, GJ ; Ren, Q.; Dilks, JR ; Blair, P.; Whiteheart, SW ; Flaumenhaft, R. "Endobrevin/VAMP-8-dependent dense granule release mediates thrombus formation in vivo." Blood 114, 5 (2009): 1083-90. [PubMed Link] | [ Full text ]
  • Konopatskaya, O.; Gilio, K.; Harper, MT ; Zhao, Y.; Cosemans, JM ; Karim, ZA ; Whiteheart, SW ; Molkentin, JD ; Verkade, P.; Watson, SP ; Heemskerk, JW ; Poole, AW "PKCalpha regulates platelet granule secretion and thrombus formation in mice." The Journal of clinical investigation 119, 2 (2009): 399-407. [PubMed Link] | [ Full text ]
  • Ren, Q.; Ye, S.; Whiteheart, SW "The platelet release reaction: just when you thought platelet secretion was simple." Current opinion in hematology 15, 5 (2008): 537-41. [PubMed Link] | [ Full text ]
  • Matveeva, EA ; Vanaman, TC ; Whiteheart, SW ; Slevin, JT "Levetiracetam prevents kindling-induced asymmetric accumulation of hippocampal 7S SNARE complexes." Epilepsia 49, 10 (2008): 1749-58. [PubMed Link] | [ Full text ]
  • Karim, ZA ; Choi, W.; Whiteheart, SW "Primary platelet signaling cascades and integrin-mediated signaling control ADP-ribosylation factor (Arf) 6-GTP levels during platelet activation and aggregation." The Journal of biological chemistry 283, 18 (2008): 11995-2003. [PubMed Link] | [ Full text ]
  • Crawford, GL ; Hart, GW ; Whiteheart, SW "Murine platelets are not regulated by O-linked beta-N-acetylglucosamine." Archives of biochemistry and biophysics 474, 1 (2008): 220-4. [PubMed Link] | [ Full text ]
  • Smyth, SS ; Monroe DM, 3rd ; Wysokinski, WE ; McBane RD, 2nd ; Whiteheart, SW ; Becker, RC ; Steinhubl, SR "Platelet activation and its patient-specific consequences." Thrombosis research 122, 4 (2008): 435-41. [PubMed Link] | [ Full text ]
  • Badol, P.; David-Dufilho, M.; Auger, J.; Whiteheart, SW ; Rendu, F. "Thiosulfinates modulate platelet activation by reaction with surface free sulfhydryls and internal thiol-containing proteins." Platelets 18, 7 (2007): 481-90. [PubMed Link] | [ Full text ]
  • Zhao, C.; Slevin, JT ; Whiteheart, SW "Cellular functions of NSF: not just SNAPs and SNAREs." FEBS letters 581, 11 (2007): 2140-9. [PubMed Link] | [ Full text ]
  • Matveeva, EA ; Vanaman, TC ; Whiteheart, SW ; Slevin, JT "Asymmetric accumulation of hippocampal 7S SNARE complexes occurs regardless of kindling paradigm." Epilepsy research 73, 3 (2007): 266-74. [PubMed Link] | [ Full text ]
  • Ren, Q.; Barber, HK ; Crawford, GL ; Karim, ZA ; Zhao, C.; Choi, W.; Wang, CC ; Hong, W.; Whiteheart, SW "Endobrevin/VAMP-8 is the primary v-SNARE for the platelet release reaction." Molecular biology of the cell 18, 1 (2007): 24-33. [PubMed Link] | [ Full text ]
  • Choi, W.; Karim, ZA ; Whiteheart, SW "Arf6 plays an early role in platelet activation by collagen and convulxin." Blood 107, 8 (2006): 3145-52. [PubMed Link] | [ Full text ]
  • Mackenzie, IR ; Baker, M.; West, G.; Woulfe, J.; Qadi, N.; Gass, J.; Cannon, A.; Adamson, J.; Feldman, H.; Lindholm, C.; Melquist, S.; Pettman, R.; Sadovnick, AD ; Dwosh, E.; Whiteheart, SW ; Hutton, M.; Pickering-Brown, SM "A family with tau-negative frontotemporal dementia and neuronal intranuclear inclusions linked to chromosome 17." Brain : a journal of neurology 129, Pt 4 (2006): 853-67. [PubMed Link] | [ Full text ]
  • Chen, X.; Matsumoto, H.; Hinck, CS ; Al-Hasani, H.; St-Denis, JF ; Whiteheart, SW ; Cushman, SW "Demonstration of differential quantitative requirements for NSF among multiple vesicle fusion pathways of GLUT4 using a dominant-negative ATPase-deficient NSF." Biochemical and biophysical research communications 333, 1 (2005): 28-34. [PubMed Link] | [ Full text ]
  • Hanson, PI ; Whiteheart, SW "AAA+ proteins: have engine, will work." Nature reviews. Molecular cell biology 6, 7 (2005): 519-29. [PubMed Link] | [ Full text ]
  • Gage, RM ; Matveeva, EA ; Whiteheart, SW ; Zastrow, M. "Type I PDZ ligands are sufficient to promote rapid recycling of G Protein-coupled receptors independent of binding to N-ethylmaleimide-sensitive factor." The Journal of biological chemistry 280, 5 (2005): 3305-13. [PubMed Link] | [ Full text ]
  • Hepp, R.; Puri, N.; Hohenstein, AC ; Crawford, GL ; Whiteheart, SW ; Roche, PA "Phosphorylation of SNAP-23 regulates exocytosis from mast cells." The Journal of biological chemistry 280, 8 (2005): 6610-20. [PubMed Link] | [ Full text ]
  • Schraw, TD ; Crawford, GL ; Ren, Q.; Choi, W.; Thurmond, DC ; Pessin, J.; Whiteheart, SW "Platelets from Munc18c heterozygous mice exhibit normal stimulus-induced release." Thrombosis and haemostasis 92, 4 (2004): 829-37. [PubMed Link] | [ Full text ]
  • Dean, WL ; Whiteheart, SW "Plasma membrane Ca(2+)-ATPase (PMCA) translocates to filopodia during platelet activation." Thrombosis and haemostasis 91, 2 (2004): 325-33. [PubMed Link] | [ Full text ]
  • Ogura, T.; Whiteheart, SW ; Wilkinson, AJ "Conserved arginine residues implicated in ATP hydrolysis, nucleotide-sensing, and inter-subunit interactions in AAA and AAA+ ATPases." Journal of structural biology 146, 1-2 (2004): 106-12. [PubMed Link] |
  • Whiteheart, SW ; Matveeva, EA "Multiple binding proteins suggest diverse functions for the N-ethylmaleimide sensitive factor." Journal of structural biology 146, 1-2 (2004): 32-43. [PubMed Link] | [ Full text ]
  • Rutledge, TW ; Whiteheart, SW "Studies of secretion using permeabilized platelets." Methods in molecular biology (Clifton, N.J.) 272, (2004): 109-20. [PubMed Link] | [ Full text ]
  • Schraw, TD ; Rutledge, TW ; Crawford, GL ; Bernstein, AM ; Kalen, AL ; Pessin, JE ; Whiteheart, SW "Granule stores from cellubrevin/VAMP-3 null mouse platelets exhibit normal stimulus-induced release." Blood 102, 5 (2003): 1716-22. [PubMed Link] | [ Full text ]
  • Puri, N.; Kruhlak, MJ ; Whiteheart, SW ; Roche, PA "Mast cell degranulation requires N-ethylmaleimide-sensitive factor-mediated SNARE disassembly." Journal of immunology (Baltimore, Md. : 1950) 171, 10 (2003): 5345-52. [PubMed Link] | [ Full text ]
  • Matveeva, EA ; Whiteheart, SW ; Slevin, JT "Accumulation of 7S SNARE complexes in hippocampal synaptosomes from chronically kindled rats." Journal of neurochemistry 84, 3 (2003): 621-4. [PubMed Link] | [ Full text ]
  • Skrzypek, E.; Myers-Morales, T.; Whiteheart, SW ; Straley, SC "Application of a Saccharomyces cerevisiae model to study requirements for trafficking of Yersinia pestis YopM in eucaryotic cells." Infection and immunity 71, 2 (2003): 937-47. [PubMed Link] | [ Full text ]
  • Schraw, TD ; Lemons, PP ; Dean, WL ; Whiteheart, SW "A role for Sec1/Munc18 proteins in platelet exocytosis." The Biochemical journal 374, Pt 1 (2003): 207-17. [PubMed Link] | [ Full text ]
  • Rutledge, TW ; Whiteheart, SW "SNAP-23 is a target for calpain cleavage in activated platelets." The Journal of biological chemistry 277, 40 (2002): 37009-15. [PubMed Link] | [ Full text ]
  • Matveeva, EA ; May, AP ; He, P.; Whiteheart, SW "Uncoupling the ATPase activity of the N-ethylmaleimide sensitive factor (NSF) from 20S complex disassembly." Biochemistry 41, 2 (2002): 530-6. [PubMed Link] | [ Full text ]
  • May, AP ; Whiteheart, SW ; Weis, WI "Unraveling the mechanism of the vesicle transport ATPase NSF, the N-ethylmaleimide-sensitive factor." The Journal of biological chemistry 276, 25 (2001): 21991-4. [PubMed Link] | [ Full text ]
  • Chen, D.; Xu, W.; He, P.; Medrano, EE ; Whiteheart, SW "Gaf-1, a gamma -SNAP-binding protein associated with the mitochondria." The Journal of biological chemistry 276, 16 (2001): 13127-35. [PubMed Link] | [ Full text ]
  • Matveeva, EA ; Whiteheart, SW ; Vanaman, TC ; Slevin, JT "Phosphorylation of the N-ethylmaleimide-sensitive factor is associated with depolarization-dependent neurotransmitter release from synaptosomes." The Journal of biological chemistry 276, 15 (2001): 12174-81. [PubMed Link] | [ Full text ]
  • Whiteheart, SW ; Schraw, T.; Matveeva, EA "N-ethylmaleimide sensitive factor (NSF) structure and function." International review of cytology 207, (2001): 71-112. [PubMed Link] |
  • Chen, D.; Lemons, PP ; Schraw, T.; Whiteheart, SW "Molecular mechanisms of platelet exocytosis: role of SNAP-23 and syntaxin 2 and 4 in lysosome release." Blood 96, 5 (2000): 1782-8. [PubMed Link] | [ Full text ]
  • Southard, RC ; Haggard, J.; Crider, ME ; Whiteheart, SW ; Cooper, RL "Influence of serotonin on the kinetics of vesicular release." Brain research 871, 1 (2000): 16-28. [PubMed Link] | [ Full text ]
  • Lemons, PP ; Chen, D.; Whiteheart, SW "Molecular mechanisms of platelet exocytosis: requirements for alpha-granule release." Biochemical and biophysical research communications 267, 3 (2000): 875-80. [PubMed Link] | [ Full text ]
  • Chen, D.; Bernstein, AM ; Lemons, PP ; Whiteheart, SW "Molecular mechanisms of platelet exocytosis: role of SNAP-23 and syntaxin 2 in dense core granule release." Blood 95, 3 (2000): 921-9. [PubMed Link] | [ Full text ]
  • Richards-Smith, B.; Novak, EK ; Jang, EK ; He, P.; Haslam, RJ ; Castle, D.; Whiteheart, SW ; Swank, RT "Analyses of proteins involved in vesicular trafficking in platelets of mouse models of Hermansky Pudlak syndrome." Molecular genetics and metabolism 68, 1 (1999): 14-23. [PubMed Link] | [ Full text ]
  • Chen, D.; Minger, SL ; Honer, WG ; Whiteheart, SW "Organization of the secretory machinery in the rodent brain: distribution of the t-SNAREs, SNAP-25 and SNAP-23." Brain research 831, 1-2 (1999): 11-24. [PubMed Link] | [ Full text ]
  • May, AP ; Misura, KM ; Whiteheart, SW ; Weis, WI "Crystal structure of the amino-terminal domain of N-ethylmaleimide-sensitive fusion protein." Nature cell biology 1, 3 (1999): 175-82. [PubMed Link] | [ Full text ]
  • Bernstein, AM ; Whiteheart, SW "Identification of a cellubrevin/vesicle associated membrane protein 3 homologue in human platelets." Blood 93, 2 (1999): 571-9. [PubMed Link] | [ Full text ]
  • Chen, D.; Whiteheart, SW "Intracellular localization of SNAP-23 to endosomal compartments." Biochemical and biophysical research communications 255, 2 (1999): 340-6. [PubMed Link] | [ Full text ]
  • He, P.; Southard, RC ; Chen, D.; Whiteheart, SW ; Cooper, RL "Role of alpha-SNAP in promoting efficient neurotransmission at the crayfish neuromuscular junction." Journal of neurophysiology 82, 6 (1999): 3406-16. [PubMed Link] | [ Full text ]
  • Matveeva, E.; Whiteheart, SW "The effects of SNAP/SNARE complexes on the ATPase of NSF." FEBS letters 435, 2-3 (1998): 211-4. [PubMed Link] | [ Full text ]
  • Minger, SL ; Geddes, JW ; Holtz, ML ; Craddock, SD ; Whiteheart, SW ; Siman, RG ; Pettigrew, LC "Glutamate receptor antagonists inhibit calpain-mediated cytoskeletal proteolysis in focal cerebral ischemia." Brain research 810, 1-2 (1998): 181-99. [PubMed Link] | [ Full text ]
  • Low, SH ; Chapin, SJ ; Wimmer, C.; Whiteheart, SW ; Kömüves, LG ; Mostov, KE ; Weimbs, T. "The SNARE machinery is involved in apical plasma membrane trafficking in MDCK cells." The Journal of cell biology 141, 7 (1998): 1503-13. [PubMed Link] | [ Full text ]
  • Leung, SM ; Chen, D.; DasGupta, BR ; Whiteheart, SW ; Apodaca, G. "SNAP-23 requirement for transferrin recycling in Streptolysin-O-permeabilized Madin-Darby canine kidney cells." The Journal of biological chemistry 273, 28 (1998): 17732-41. [PubMed Link] |
  • Colombo, MI ; Gelberman, SC ; Whiteheart, SW ; Stahl, PD "N-ethylmaleimide-sensitive factor-dependent alpha-SNAP release, an early event in the docking/fusion process, is not regulated by Rab GTPases." The Journal of biological chemistry 273, 3 (1998): 1334-8. [PubMed Link] |
  • Lenzen, CU ; Steinmann, D.; Whiteheart, SW ; Weis, WI "Crystal structure of the hexamerization domain of N-ethylmaleimide-sensitive fusion protein." Cell 94, 4 (1998): 525-36. [PubMed Link] | [ Full text ]
  • Matveeva, EA ; He, P.; Whiteheart, SW "N-Ethylmaleimide-sensitive fusion protein contains high and low affinity ATP-binding sites that are functionally distinct." The Journal of biological chemistry 272, 42 (1997): 26413-8. [PubMed Link] |
  • Lemons, PP ; Chen, D.; Bernstein, AM ; Bennett, MK ; Whiteheart, SW "Regulated secretion in platelets: identification of elements of the platelet exocytosis machinery." Blood 90, 4 (1997): 1490-500. [PubMed Link] | [ Full text ]
  • Timmers, KI ; Clark, AE ; Omatsu-Kanbe, M.; Whiteheart, SW ; Bennett, MK ; Holman, GD ; Cushman, SW "Identification of SNAP receptors in rat adipose cell membrane fractions and in SNARE complexes co-immunoprecipitated with epitope-tagged N-ethylmaleimide-sensitive fusion protein." The Biochemical journal 320 ( Pt 2), (1996): 429-36. [PubMed Link] | [ Full text ]
  • Colombo, MI ; Taddese, M.; Whiteheart, SW ; Stahl, PD "A possible predocking attachment site for N-ethylmaleimide-sensitive fusion protein. Insights from in vitro endosome fusion." The Journal of biological chemistry 271, 31 (1996): 18810-6. [PubMed Link] |
  • Apodaca, G.; Cardone, MH ; Whiteheart, SW ; DasGupta, BR ; Mostov, KE "Reconstitution of transcytosis in SLO-permeabilized MDCK cells: existence of an NSF-dependent fusion mechanism with the apical surface of MDCK cells." The EMBO journal 15, 7 (1996): 1471-81. [PubMed Link] | [ Full text ]
  • DeBello, WM ; O'Connor, V.; Dresbach, T.; Whiteheart, SW ; Wang, SS ; Schweizer, FE ; Betz, H.; Rothman, JE ; Augustine, GJ "SNAP-mediated protein-protein interactions essential for neurotransmitter release." Nature 373, 6515 (1995): 626-30. [PubMed Link] | [ Full text ]
  • Whiteheart, SW ; Kubalek, EW "SNAPs and NSF: general members of the fusion apparatus." Trends in cell biology 5, 2 (1995): 64-8. [PubMed Link] | [ Full text ]
  • Nagiec, EE ; Bernstein, A.; Whiteheart, SW "Each domain of the N-ethylmaleimide-sensitive fusion protein contributes to its transport activity." The Journal of biological chemistry 270, 49 (1995): 29182-8. [PubMed Link] |
  • Burns, ME ; Beushausen, SA ; Chin, GJ ; Tang, D.; DeBello, WM ; Dresbach, T.; O'Connor, V.; Schweizer, FE ; Wang, SS ; Whiteheart, SW "Proteins involved in synaptic vesicle docking and fusion." Cold Spring Harbor symposia on quantitative biology 60, (1995): 337-48. [PubMed Link] | [ Full text ]
  • Whiteheart, SW ; Hart, GW "Incorporation of 3H ethanolamine into a single cytosolic protein in a cell free system: ethanolaminylation of EF-1 alpha in vitro." Archives of biochemistry and biophysics 309, 2 (1994): 387-91. [PubMed Link] | [ Full text ]
  • Whiteheart, SW ; Rossnagel, K.; Buhrow, SA ; Brunner, M.; Jaenicke, R.; Rothman, JE "N-ethylmaleimide-sensitive fusion protein: a trimeric ATPase whose hydrolysis of ATP is required for membrane fusion." The Journal of cell biology 126, 4 (1994): 945-54. [PubMed Link] | [ Full text ]
  • Söllner, T.; Bennett, MK ; Whiteheart, SW ; Scheller, RH ; Rothman, JE "A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of synaptic vesicle docking, activation, and fusion." Cell 75, 3 (1993): 409-18. [PubMed Link] | [ Full text ]
  • Whiteheart, SW ; Griff, IC ; Brunner, M.; Clary, DO ; Mayer, T.; Buhrow, SA ; Rothman, JE "SNAP family of NSF attachment proteins includes a brain-specific isoform." Nature 362, 6418 (1993): 353-5. [PubMed Link] | [ Full text ]
  • Söllner, T.; Whiteheart, SW ; Brunner, M.; Erdjument-Bromage, H.; Geromanos, S.; Tempst, P.; Rothman, JE "SNAP receptors implicated in vesicle targeting and fusion." Nature 362, 6418 (1993): 318-24. [PubMed Link] | [ Full text ]
  • Wilson, DW ; Whiteheart, SW ; Wiedmann, M.; Brunner, M.; Rothman, JE "A multisubunit particle implicated in membrane fusion." The Journal of cell biology 117, 3 (1992): 531-8. [PubMed Link] | [ Full text ]
  • Whiteheart, SW ; Brunner, M.; Wilson, DW ; Wiedmann, M.; Rothman, JE "Soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAPs) bind to a multi-SNAP receptor complex in Golgi membranes." The Journal of biological chemistry 267, 17 (1992): 12239-43. [PubMed Link] |
  • Wilson, DW ; Whiteheart, SW ; Orci, L.; Rothman, JE "Intracellular membrane fusion." Trends in biochemical sciences 16, 9 (1991): 334-7. [PubMed Link] |
  • Whiteheart, SW ; McLenithan, JC ; Hart, GW "Surfaces of murine lymphocyte subsets differ in sialylation states and antigen distribution of a major N-linked penultimate saccharide structure." Cellular immunology 125, 2 (1990): 337-53. [PubMed Link] | [ Full text ]
  • Whiteheart, SW ; Shenbagamurthi, P.; Chen, L.; Cotter, RJ ; Hart, GW "Murine elongation factor 1 alpha (EF-1 alpha) is posttranslationally modified by novel amide-linked ethanolamine-phosphoglycerol moieties. Addition of ethanolamine-phosphoglycerol to specific glutamic acid residues on EF-1 alpha." The Journal of biological chemistry 264, 24 (1989): 14334-41. [PubMed Link] |
  • Whiteheart, SW ; Passaniti, A.; Reichner, JS ; Holt, GD ; Haltiwanger, RS ; Hart, GW "Glycosyltransferase probes." Methods in enzymology 179, (1989): 82-95. [PubMed Link] | [ Full text ]
  • Reichner, JS ; Whiteheart, SW ; Hart, GW "Intracellular trafficking of cell surface sialoglycoconjugates." The Journal of biological chemistry 263, 31 (1988): 16316-26. [PubMed Link] |
  • Whiteheart, SW ; Hart, GW "Sialyltransferases as specific cell surface probes of terminal and penultimate saccharide structures on living cells." Analytical biochemistry 163, 1 (1987): 123-35. [PubMed Link] | [ Full text ]
  • Powell, LD ; Whiteheart, SW ; Hart, GW "Cell surface sialic acid influences tumor cell recognition in the mixed lymphocyte reaction." Journal of immunology (Baltimore, Md. : 1950) 139, 1 (1987): 262-70. [PubMed Link] | [ Full text ]
  • Campbell, RD ; Love, SH ; Whiteheart, SW ; Young, B.; Myrvik, QN "Increased hyaluronic acid is associated with dermal delayed-type hypersensitivity." Inflammation 6, 3 (1982): 235-44. [PubMed Link] |
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